Evaluation of the Accusport® Lactate Analyser

It has been suggested that lactate concentrations may provide a guide to an optimal training intensity. However, lactate concentrations established during incremental exercise in the laboratory are not always indicative of what is occurring during constant-load exercise at the same intensity. Ideally, lactate concentrations should be measured during a training session and immediately reported to the athlete to ensure that the athlete is working at the desired intensity. The purpose of this investigation was, therefore, to determine the reliability and validity of a compact, portable lactate analyser (ACCUSPORT; Boeringer Mannheim, Castle Hill, Australia). A total of 224 capillary blood samples were taken from athletes who took part in routine laboratory testing. Seventy-three of these capillary blood samples were analysed in duplicate with the Accusport for determination of intraclass, single-trial reliability. Day-to-day intraclass reliability of the Accusport was assessed by analyzing known concentrations of aqueous lactate solutions on seven consecutive days. The validity of the Accusport analyser was assessed by comparing the 224 capillary blood lactate concentrations determined on the Accusport with the lactate concentration obtained using a MICRO STAT LM3 (Analox Instruments Ltd., London, UK). In addition, lactate parameters derived from the lactate concentrations obtained with the two analysers were compared. The Accusport showed high single-trial intraclass reliability (R = 0.992; Standard Error of Measurement [SE_M] = 0.3 mmol × l^-1; n = 73) and high day-to-day intraclass reliability (R = 0.993; SE_M = 0.4 mmol × l^-1; n = 42). Despite a strong correlation between blood lactate concentrations obtained on the two analysers (r = 0.96; n = 224) the limits of agreement were + 1.9 to - 2.2 mmol × l^-1. Although the mean values for power output, HR and lactate concentration associated with the lactate parameters were not significantly different when determined on the Accusport or Micro Stat, some individuals did record large differences between analysis methods. In summary, the results of this investigation have shown that lactate concentrations can be reliably determined within a single trial and from day-to-day using the Accusport analyser. However, for some athletes, it is not valid to compare lactate concentrations or lactate parameters determined on the Accusport with those determined using the Micro Stat LM3 lactate analyser.

Key words:

Micro Stat, Dmax, lactate thresholds, reliability, validity.

References

• 1 Allen W K, Seals D R, Hurley B, Ehsani A, Hagberg J M. Lactate threshold and distance running performance in young and older endurance athletes.  J Appl Physiol. 1985;  58 1281-1284
• 2 Bishop D, Jenkins D G, Mackinnon L T. The relationship between plasma lactate parameters, W_peak and 1 h cycling performance in women.  Med Sci Sports Exerc. 1998;  30 1270-1275
• 3 Bland J M, Altman D G. Comparing two methods of clinical measurement: A personal history.  International Journal of Epidemiology. 1995;  24 S7-S14
• 4 Cheng B, Kuipers H, Snyder A C, Keizer H A, Jeukendrup A, Hesselink M. A new approach for the determination of ventilatory and lactate thresholds.  Int J Sports Med. 1992;  13 518-522
• 5 Clough M, Martin D T, Pyne D, Kearns A, Daley P, Lee H, Ryan R, Ashenden M, Hahn A. Interpretation of accusport blood lactate results - how do they compare with YSI2300 stat values (abstract). The Australian Conference of Science and Medicine in Sport 1997: 82-83
• 6 Coen B, Schwarz L, Urhausen A, Kindermann W. Control of training in middle- and long-distance running by means of the individual anaerobic threshold.  Int J Sports Med. 1991;  12 519-524
• 7 Farrell P A, Wilmore J H, Coyle E F, Billing J E, Costill D L. Plasma lactate accumulation and distance running performance.  Med Sci Sports Exerc. 1979;  11 338-344
• 8 Fell J W, Rayfield J M, Gulbin J P, Gaffney P T. Evaluation of the Accusport Lactate Analyser.  Int J Sports Med. 1998;  19 199-204
• 9 Fohrenbach R, Mader A, Hollman W. Determination of endurance capacity and prediction of exercise intensities for training and competition in marathon runners.  Int J Sports Med. 1987;  8 11-18
• 10 Foxdal P, Sjodin A, Sjodin B. Comparison of blood lactate concentrations obtained during incremental and constant intensity exercise.  Int J Sports Med. 1996;  17 360-365
• 11 Foxdal P, Sjodin B, Sjodin A, Ostman B. The validity and accuracy of blood lactate measurements for prediction of maximal endurance running capacity.  Int J Sports Med. 1994;  15 89-95
• 12 Heck H, Mader A, Hess G, Mucke S, Muller R, Hollman W. Justification of the 4 mmol/l lactate threshold.  Int J Sports Med. 1985;  6 117-130
• 13 Henritze J, Weltman A, Schurrer R L, Barlow K. Effects of training at and above the lactate threshold on the lactate threshold and maximal oxygen uptake.  Eur J Appl Physiol. 1985;  54 84-88
• 14 Jacobs I. Blood lactate: Implications for training and sports performance.  Sports Med. 1986;  3 10-25
• 15 Keith S P, Jacobs I, McLellan T M. Adaptations to training at the individual anaerobic threshold.  Eur J Appl Physiol. 1992;  65 316-323
• 16 Kindermann W, Simon G, Keul J. The significance of the aerobic-anaerobic transition for the determination of work load intensities during endurance training.  Eur J Appl Physiol. 1979;  42 25-34
• 17 Kirkendall D R, Gruber J J, Johnson R E. Measurement and evaluation for physical educators. Champaign, IL; Human Kinetics 1987
• 18 Kumagai S, Tanaka K, Matsura Y, Hirakoba K, Asano K. Relationships of the anaerobic threshold with 5 km, 10 km ad 10 mile races.  Eur J Appl Physiol. 1982;  54 58-61
• 19 Lehmann M, Berg A, Kapp R, Wessinhage T, Keul J. Correlations between laboratory testing and distance running performance in marathoners of similar performance ability.  Int J Sports Med. 1983;  4 226-230
• 20 Lindner A. Measurement of plasma lactate concentration with Accusport.  Equine Vet J. 1996;  28 403-405
• 21 Mikesell K A, Dudley G A. Influence of intense endurance training on aerobic power of competitive distance runners.  Med Sci Sports Exerc. 1984;  16 371-375
• 22 Mognoni P, Sirtori M D, Lorenzelli F, Cerretelli P. Physiological responses during prolonged exercise at the power output corresponding to the blood lactate threshold.  Eur J Appl Physiol. 1990;  60 239-243
• 23 Naik J, Snyder A C, Welsh R, Hyatt K. Validity and reliability of accusport lactate monitor.  Med Sci Sports Exerc. 1996;  28 S391
• 24 Orok C J, Hughson R L, Green H J, Thomson J A. Blood lactate responses in incremental exercise as predictors of constant load performance.  Eur J Appl Physiol. 1989;  59 262-267
• 25 Oyono-Enguelle S, Heitz A, Marbach J, Ott C, Gartner M, Pape A, Vollmer J C, Freund H. Blood lactate during constant-load exercise at aerobic and anaerobic thresholds.  Eur J Appl Physiol. 1990;  60 321-330
• 26 Poole D C, Gaesser G A. Response of ventilatory and lactate thresholds to continuous and interval training.  J Appl Physiol. 1985;  58 1115-1121
• 27 Safrit M J, Wood T M. Measurement concepts in physical education and exercise science. Champaign, IL; Human Kinetics 1989: 62
• 28 Sjodin B, Jacobs I. Onset of blood lactate accumulation and marathon running performance.  Int J Sports Med. 1981;  2 23-26
• 29 Thoden J. Testing aerobic power. In: MacDougall JD, Wenger HA, Green HJ (eds) Physiological testing of the high-performance athlete. Champaign, IL; Human Kinetics Books 1991 2nd edition: 107-174
• 30 Vincent W J. Statistics in Kinesiology. Champaign, IL; Human Kinetics 1995: 178-181
• 31 Warych K L, Sabina T E, Moreau K L, Fischer K C, Kaminsky L A, Whaley M H. Evaluation of the accusport blood lactate monitor.  Med Sci Sports Exerc. 1996;  28 S1173
• 32 Weltman A, Seip R L, Snead D, Weltman J Y, Haskvitz E M, Evans W S, Velduis J D, Rogol A D. Exercise training at and above the lactate threshold in previously untrained women.  Int J Sports Med. 1992;  13 257-263
• 33 Wigglesworth J K, LaMere V J, Rowland N D, Miller L. Examination of the reliability of a new blood lactate analyser.  Med Sci Sports Exerc. 1996;  28 S59
• 34 Yoshida T, Chida M, Ichioka M, Suda Y. Blood lactate parameters related to aerobic capacity and endurance performance.  Eur J Appl Physiol. 1987;  56 7-11
• 35 Yoshida T, Suda Y, Takeuchi N. Endurance training regimen based upon arterial blood lactate: effects on anaerobic threshold.  Eur J Appl Physiol. 1982;  49 223-230