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Planta Med 2001; 67(3): 224-229
DOI: 10.1055/s-2001-11991
Original Paper
Pharmacology
© Georg Thieme Verlag Stuttgart · New York

Mechanisms of Relaxant Action of S-Petasin and S-Isopetasin, Sesquiterpenes of Petasites formosanus, in Isolated Guinea Pig Trachea

Wun-Chang Ko1,*, Chien-Bang Lei1 , Yun-Lian Lin2 , Chieh-Fu Chen2
  • 1 Graduate Institute of Medical Sciences, Taipei Medical College, Taipei, Taiwan, R.O.C.
  • 2 National Research Institute of Chinese Medicine, Taipei, Taiwan, R.O.C.
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Publikationsverlauf

March 7, 2000

July 3, 2000

Publikationsdatum:
31. Dezember 2001 (online)

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Abstract

We investigated the mechanisms of action of S-petasin and S-isopetasin, from Petasites formosanus Kitamura which is used as a folk medicine for treating hypertension, tumors, and asthma in Taiwan. The tension changes of tracheal segments were isometrically recorded on a polygraph. S-Petasin and S-isopetasin non-competitively inhibited cumulative histamine-, and carbachol-induced contractions with an exception that S-isopetasin produced a parallel, rightward shift of the concentration-response curve of carbachol in a competitive manner. S-Petasin also non-competitively inhibited cumulative Ca2+-induced contractions in depolarized (K+, 60 mM; histamine, 100 μM; or carbachol, 10 μM) guinea-pig tracheas. S-Isopetasin did in depolarized (K+, 60 mM) trachea too. The nifedipine (10 μM)-remaining tension of carbachol (0.2 μM)-induced precontraction was further relaxed by S-petasin or S-isopetasin, suggesting that no matter whether either blocked VDCCs or not, S-petasin or S-isopetasin may have other mechanisms of relaxant action. The relaxant effect of S-petasin or S-isopetasin was unaffected by the presence of propranolol (1 μM), 2′,5′-dideoxyadenosine (10 μM), methylene blue (25 μM), glibenclamide (10 μM), N ω-nitro-L-arginine (20 μM), or α-chymotrypsin (1 U/ml). However, S-petasin (100 - 300 μM), but not S-isopetasin, significantly inhibited cAMP-, but not cGMP-dependent PDE activity of the trachealis. The above results reveal that the mechanisms of relaxant action of S-petasin and S-isopetasin may be primarily due to its non-specific antispasmodic and antimuscarinic effects, respectively.

Abbreviations

ROCCs:receptor-operated calcium channels

VDCCs:voltage dependent calcium channels

cAMP:adenosine 3′,5′-cyclic monophosphate

cGMP:guanosine 3′,5′-cyclic monophosphate

PDE:phosphodiesterase

IBMX:3-isobutyl-1-methylxanthine

Key words

S-Petasin - S-isopetasin - Petasites formosanus - Asteraceae - guinea-pig trachea - calcium release - calcium influx - cAMP-dependent PDE

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Prof. Wun-Chang Ko

Graduate Institute of Medical Sciences

Taipei Medical College

250 Wu-Hsing St.

Taipei 110

Taiwan, R.O.C.

eMail: wc_ko@tmc.edu.tw

Fax: +886-2-2377-7639