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Plant Biol (Stuttg) 2000; 2(3): 297-305
DOI: 10.1055/s-2000-3698
Original Paper
Georg Thieme Verlag Stuttgart ·New York

RAPD Analysis Reveals Geographic Differentiations within Allium schoenoprasum L. (Alliaceae)

N. Friesen 1,2 , F. R. Blattner 1
  • 1 Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany
  • 2 John Innes Centre, Norwich NR7 4UH, UK
Weitere Informationen

Publikationsverlauf

July 29, 1999

February 1, 2000

Publikationsdatum:
31. Dezember 2000 (online)

   Lizenzen und Reprints

Abstract

Random amplified polymorphic DNA (RAPD) analysis was used to study the phylogenetic relationships between species in Allium section Schoenoprasum and for the investigation of the intraspecific differentiation of A. schoenoprasum. RAPD analysis of 39 samples representing eight species of sect. Schoenoprasum and one sample of A. atrosanguineum (sect. Annuloprasum) resulted in 233 interpretable RAPD bands. The analysis clearly distinguishes the species of section Schoenoprasum. The arrangement of the accessions of A. schoenoprasum in all dendrograms mirrors the geographical distribution, with a clear differentiation between an Asian and European subgroup. Within the European group, Scandinavian material is clearly distinct from S and E European material. Informally described morphological types of A. schoenoprasum could not be confirmed by RAPD analysis but represent recurrent ecological adaptations. A combination of phenetic (UPGMA, neighbour-joining analysis), cladistic (parsimony analysis), and statistical (PCA) methods of data analysis resulted in clearer phylogenetic interpretations than each of the methods facilitates when used separately.

Abbreviations

RAPD: random amplified polymorphic DNA

UPGMA: unweighted pair group method using arithmetic averages

NJ: neighbour-joining analysis

MP: maximum parsimony

PCA: principal coordinate analysis

Key words

Allium sect. Schoenoprasum - Alliaceae - biogeography - ecology - evolution - RAPD

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 N. Friesen

John Innes Centre

Norwich NR7 4UH UK

eMail: nikolai.friesen@bbsrc.ac.uk

Section Editor: M. Hasebe

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