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DOI: 10.1055/s-0044-100723
Green Quantification Strategy Combined with Chemometric Analysis for Triglycerides in Seeds Used in Traditional Chinese Medicine
Publication History
received 29 May 2017
revised 26 December 2017
accepted 03 January 2018
Publication Date:
31 January 2018 (online)
![](https://www.thieme-connect.de/media/plantamedica/20180607/lookinside/thumbnails/10-1055-s-0044-100723_pmb0473-1.jpg)
Abstract
Triglycerides are the primary constituents of some seed kernels used in traditional Chinese medicine. Quality control of seed kernels containing multiple components with an environmentally friendly method is indispensable for establishing their quality standards (called monographs) in pharmacopeia. Using coix seeds (Semen Coicis) as an example, a green quantification strategy was proposed by combining C8 core-shell particles with single standard to determine multicomponent technologies to quantify seven triglycerides simultaneously. A core-shell column, namely, Halo C8 (3.0 × 100 mm, 2.7 µm), was used. Methanol was used as the mobile phase at a flow rate of 0.3 mL/min, enabling UV detection of the elutes. Seven triglycerides were well separated in 20 min, and simultaneously quantified using triolein as a single standard. The conversion factor for each standard was set as 1.0 on ELSD, while for the conversion factors at 203 nm, the values increased with the reduction of linoleate. The recovery values were all in the range of 97 – 107% (RSD < 3.0%). The RSD values of precision, including intraday and intermediate precision, were < 3.0% when the total content of triglycerides was calculated. The linearity reached r ≥ 0.9990, and the limit of quantitation reached 40 – 70 ng. Forty-nine batches of coix seeds from four different places of origins and eight batches of adulterants were evaluated and differentiated using principal component analysis. In addition, the validated method was used successfully to quantity seven triglycerides in Semen Persicae, Semen Armeniacae Amarum, and Semen Pruni.
Key words
triglycerides - single standard to determine multicomponents - Coix lacryma-jobi var. ma-yuen - Poaceae - triolein - core-shell - chemometrics* These two authors contributed equally to this work.
Supporting Information
- Supporting Information
Detailed results of method validation and the quantification of all samples are available as Supporting Information.
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