PDF herunterladen
Drug Res (Stuttg) 2018; 68(06): 335-343
DOI: 10.1055/s-0043-121338
Original Article
© Georg Thieme Verlag KG Stuttgart · New York

Synergistic Effect of Free and Nano-encapsulated Chrysin-Curcumin on Inhibition of hTERT Gene Expression in SW480 Colorectal Cancer Cell Line

Raana Bagheri
1   Department of Molecular Genetics, Islamic Azad University, Tabriz, Iran
,
Zohreh Sanaat
2   Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
,
Nosratollah Zarghami
2   Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
3   Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
4   Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
› Institutsangaben
Weitere Informationen

Publikationsverlauf

Publikationsdatum:
02. Januar 2018 (online)

Auch verfügbar auf
    Lizenzen und Reprints

Erratum zu diesem Artikel:

Correction: Synergistic Effect of Free and Nano-encapsulated Chrysin-Curcumin on Inhibition of hTERT Gene Expression in SW480 Colorectal Cancer Cell LineDrug Res (Stuttg) 2018; 68(06): e2-e2
DOI: 10.1055/s-0037-1600990

Abstract

Background Telomerase is known as a global therapeutic target in cancer cells due to its main role in tumorigenesis. Nowadays, it is proposed new treatment methods based on molecular target therapy by bioactive substances such as curcumin and chrysin with fewer side effects than other chemical drugs. But due to their low aqueous solubility and high clearance in the bloodstream it can be used of nanoparticles to increase their half-life and biocompatibility of them. Therefore, the goal of this study was to evaluate the effect of Chrysin-Curcumin on the expression of telomerase gene in SW480 colorectal cancer cell line.

Material and method PLGA-PEG nanoparticles synthesized and were confirmed using by the scanning electron microscope (SEM) and FTIR Spectroscopy. After treatment of SW480 cells by curcumin and chrysin loaded nanoparticles, their toxicity to cancer cells, was evaluated by MTT. Then, the inhibition of hTERT gene expression was measured using qRT-PCR method.

Result The results of MTT test showed nanocapsulated curcumin and chrysin compared with free forms of these compounds have high synergistic effect on sw480 cells. Also, real time-PCR showed significant decrease in hTERT gene expression in SW480 cells that treated with nano-curcumin and nano-chrysin compare to untreated cells.

Conclusion Nano-encapsulation of curcumin and chrysin enhanced delivery of these compounds to SW480 colorectal cancer cells and therefore it can be conclude that PLGA-PEG nanoparticles promote anticancer effects of curcumin-chrysin by increasing bioavailability and the solubility of these drugs.

Key words

Curcumin - Chrysin - PLGA-PEG - Nanoparticle - Telomerase - Colorectal Cancer
 

  • References

  • 1 Kirkøen B, Berstad P, Botteri E et al. Acceptability of two colorectal cancer screening tests: pain as a key determinant in sigmoidoscopy. Endoscopy 2017
    PubMed
  • 2 Durko L, Malecka-Panas E. Lifestyle modifications and colorectal cancer. Current Colorectal Cancer Reports 2014; 10: 45-54
    CrossrefPubMedSuche in Google Scholar
  • 3 Montazeri M, Sadeghizadeh M, Pilehvar-Soltanahmadi Y. et al. Dendrosomal curcumin nanoformulation modulate apoptosis-related genes and protein expression in hepatocarcinoma cell lines. International journal of pharmaceutics 2016; 509: 244-254
    CrossrefPubMedSuche in Google Scholar
  • 4 Fekri Aval S, Akbarzadeh A, Yamchi MR. et al. Gene silencing effect of SiRNA-magnetic modified with biodegradable copolymer nanoparticles on hTERT gene expression in lung cancer cell line. Artificial cells, Nanomedicine, and Biotechnology 2016; 44: 188-193
    CrossrefPubMedSuche in Google Scholar
  • 5 Sheervalilou R, Ansarin K, Fekri Aval S. et al. An update on sputum MicroRNAs in lung cancer diagnosis. Diagnostic Cytopathology 2016; 44: 442-449
    CrossrefPubMedSuche in Google Scholar
  • 6 Zavari-Nematabad A, Alizadeh-Ghodsi M, Hamishehkar H. et al. Development of quantum-dot-encapsulated liposome-based optical nanobiosensor for detection of telomerase activity without target amplification. Analytical and bioanalytical chemistry 2017; 409: 1301-1310
    CrossrefPubMedSuche in Google Scholar
  • 7 Panahi Y, Mohammadhosseini M, Nejati-Koshki K. et al. Preparation, surface properties, and therapeutic applications of gold nanoparticles in biomedicine. Drug research 2017; 67: 77-87
    Thieme ConnectPubMedSuche in Google Scholar
  • 8 Ghalhar MG, Akbarzadeh A, Rahmati M. et al. Comparison of inhibitory effects of 17-AAG nanoparticles and free 17-AAG on HSP90 gene expression in breast cancer. Asian Pac J Cancer Prev 2014; 15: 7113-7118
    CrossrefPubMedSuche in Google Scholar
  • 9 Javidfar S, Pilehvar-Soltanahmadi Y, Farajzadeh R et al. The inhibitory effects of nano-encapsulated metformin on growth and hTERT expression in breast cancer cells. Journal of Drug Delivery Science and Technology 2017
    PubMed
  • 10 Lotfi-Attari J, Pilehvar-Soltanahmadi Y, Dadashpour M. et al. Co-Delivery of Curcumin and Chrysin by Polymeric Nanoparticles Inhibit Synergistically Growth and hTERT Gene Expression in Human Colorectal Cancer Cells. Nutrition and Cancer 2017; 69: 1-10
    CrossrefPubMedSuche in Google Scholar
  • 11 Rami A, Zarghami N. Comparison of inhibitory effect of curcumin nanoparticles and free curcumin in human telomerase reverse transcriptase gene expression in breast cancer. Advanced Pharmaceutical Bulletin 2013; 3: 127
    PubMedSuche in Google Scholar
  • 12 Mohammad P, Nosratollah Z, Mohammad R. et al. The inhibitory effect of Curcuma longa extract on telomerase activity in A549 lung cancer cell line. African Journal of Biotechnology 2010; 9: 912-919
    CrossrefPubMedSuche in Google Scholar
  • 13 Badrzadeh F, Akbarzadeh A, Zarghami N. et al. Comparison between effects of free curcumin and curcumin loaded NIPAAm-MAA nanoparticles on telomerase and PinX1 gene expression in lung cancer cells. Asian Pac J Cancer Prev 2014; 15: 8931-8936
    CrossrefPubMedSuche in Google Scholar
  • 14 Santos IS, Ponte BM, Boonme P. et al. Nanoencapsulation of polyphenols for protective effect against colon–rectal cancer. Biotechnology Advances 2013; 31: 514-523
    CrossrefPubMedSuche in Google Scholar
  • 15 Deldar Y, Pilehvar-Soltanahmadi Y, Dadashpour M. et al. An in vitro examination of the antioxidant, cytoprotective and anti-inflammatory properties of chrysin-loaded nanofibrous mats for potential wound healing applications. Artificial Cells, Nanomedicine, and Biotechnology 2017; 1-11
    PubMedSuche in Google Scholar
  • 16 Deldar Y, Zarghami F, Pilehvar-Soltanahmadi Y. et al. Antioxidant effects of chrysin-loaded electrospun nanofibrous mats on proliferation and stemness preservation of human adipose-derived stem cells. Cell and Tissue Banking 2017; 1-13
    PubMedSuche in Google Scholar
  • 17 Eatemadi A, Daraee H, Aiyelabegan HT. et al. Synthesis and characterization of chrysin-loaded PCL-PEG-PCL nanoparticle and its effect on breast cancer cell line. Biomedicine & Pharmacotherapy 2016; 84: 1915-1922
    CrossrefPubMedSuche in Google Scholar
  • 18 Ronnekleiv-Kelly SM, Nukaya M, Díaz-Díaz CJ. et al. Aryl hydrocarbon receptor-dependent apoptotic cell death induced by the flavonoid chrysin in human colorectal cancer cells. Cancer Letters 2016; 370: 91-99
    CrossrefPubMedSuche in Google Scholar
  • 19 Mohammadian F, Abhari A, Dariushnejad H. et al. Effects of chrysin-PLGA-PEG nanoparticles on proliferation and gene expression of miRNAs in gastric cancer cell line. Iranian Journal of Cancer Prevention 2016; 9: e4190
    PubMedSuche in Google Scholar
  • 20 Mohammadi Z, Zak MS, Seidi K. et al. The Effect of chrysin Loaded PLGA-PEG on metalloproteinase gene expression in mouse 4t1 tumor model. Drug Research 2017; 67: 211-216
    Thieme ConnectPubMedSuche in Google Scholar
  • 21 Ghosh S, Banerjee S, Sil PC. The beneficial role of curcumin on inflammation, diabetes and neurodegenerative disease: A recent update. Food and Chemical Toxicology 2015; 83: 111-124
    CrossrefPubMedSuche in Google Scholar
  • 22 Alibakhshi A, Ranjbari J, Pilehvar-Soltanahmadi Y. et al. An update on phytochemicals in molecular target therapy of cancer: Potential inhibitory effect on telomerase activity. Current Medicinal Chemistry 2016; 23: 2380-2393
    CrossrefPubMedSuche in Google Scholar
  • 23 Anganeh MT, Mirakabad FST, Izadi M. et al. The comparison between effects of free curcumin and curcumin loaded PLGA-PEG on telomerase and TRF1 expressions in calu-6 lung cancer cell line. Int J Biosci 2014; 4: 134-145
    PubMedSuche in Google Scholar
  • 24 Nejati-Koshki K, Akbarzadeh A, Pourhassan-Moghaddam M. Curcumin inhibits leptin gene expression and secretion in breast cancer cells by estrogen receptors. Cancer Cell International 2014; 14: 66
    CrossrefPubMedSuche in Google Scholar
  • 25 Zeighamian V, Darabi M, Akbarzadeh A. et al. PNIPAAm-MAA nanoparticles as delivery vehicles for curcumin against MCF-7 breast cancer cells. Artificial cells, Nanomedicine, and Biotechnology 2016; 44: 735-742
    CrossrefPubMedSuche in Google Scholar
  • 26 Pourhassan-Moghaddam M, Zarghami N, Mohsenifar A. et al. Watercress-based gold nanoparticles: biosynthesis, mechanism of formation and study of their biocompatibility in vitro. Micro & Nano Letters 2014; 9: 345-350
    CrossrefPubMedSuche in Google Scholar
  • 27 Montazeri M, Pilehvar-Soltanahmadi Y, Mohaghegh M. et al. Antiproliferative and Apoptotic Effect of Dendrosomal Curcumin Nanoformulation in P53 Mutant and Wide-Type Cancer Cell Lines. Anti-Cancer Agents in Medicinal Chemistry (Formerly Current Medicinal Chemistry-Anti-Cancer Agents) 2017; 17: 662-673
    CrossrefPubMedSuche in Google Scholar
  • 28 Sadeghzadeh H, Pilehvar-Soltanahmadi Y, Akbarzadeh A. et al. The Effects of Nanoencapsulated Curcumin-Fe3O4 on Proliferation and hTERT Gene Expression in Lung Cancer Cells. Anti-cancer Agents in Medicinal Chemistry 2017; 17: 1363-1373
    PubMedSuche in Google Scholar
  • 29 Anari E, Akbarzadeh A, Zarghami N. Chrysin-loaded PLGA-PEG nanoparticles designed for enhanced effect on the breast cancer cell line. Artificial cells, Nanomedicine, And Biotechnology 2016; 44: 1410-1416
    CrossrefPubMedSuche in Google Scholar
  • 30 Tabatabaei Mirakabad FS, Akbarzadeh A, Milani M. et al. A Comparison between the cytotoxic effects of pure curcumin and curcumin-loaded PLGA-PEG nanoparticles on the MCF-7 human breast cancer cell line. Artificial cells, nanomedicine, and biotechnology 2016; 44: 423-430
    CrossrefPubMedSuche in Google Scholar
  • 31 Makadia HK, Siegel SJ. Poly lactic-co-glycolic acid (PLGA) as biodegradable controlled drug delivery carrier. Polymers 2011; 3: 1377-1397
    CrossrefPubMedSuche in Google Scholar
  • 32 Mohammadian F, Abhari A, Dariushnejad H. et al. Upregulation of Mir-34a in AGS gastric cancer cells by a PLGA-PEG-PLGA chrysin nano formulation. Asian Pac J Cancer Prev 2015; 16: 8259-8263
    PubMedSuche in Google Scholar
  • 33 Dwivedi M, Sharma S, Shukla P. et al. Rawat AKS. Development and evaluation of anticancer polymeric nano-formulations containing curcumin and natural bioenhancers. Journal of Biomaterials and Tissue Engineering 2014; 4: 198-202
    CrossrefPubMedSuche in Google Scholar
  • 34 Braden AR, Vishwanatha JK, Kafka E. Formulation of active agent loaded activated plga nanoparticles for targeted cancer nano-therapeutics. Google Patents 2015
    PubMed
  • 35 Tong R, Langer R. Nanomedicines targeting the tumor microenvironment. The Cancer Journal 2015; 21: 314-321
    CrossrefPubMedSuche in Google Scholar
  • 36 Mohammadian F, Pilehvar-Soltanahmadi Y, Zarghami F. et al. Upregulation of miR-9 and Let-7a by nanoencapsulated chrysin in gastric cancer cells. Artificial cells, Nanomedicine, and Biotechnology 2017; 45: 1201-1206
    CrossrefPubMedSuche in Google Scholar
  • 37 Piwowarski JP, Granica S, Kiss AK. Lythrum salicaria L.—Underestimated medicinal plant from European traditional medicine. A review. Journal of Ethnopharmacology 2015; 170: 226-250
    CrossrefPubMedSuche in Google Scholar
  • 38 Mohammadian F, Pilehvar-Soltanahmadi Y, Alipour S. et al. Chrysin Alters microRNAs expression levels in gastric cancer cells: Possible molecular mechanism. Drug Research 2017; 67: 509-514
    Thieme ConnectPubMedSuche in Google Scholar
  • 39 Kazemi-Lomedasht F, Rami A, Zarghami N. Comparison of inhibitory effect of curcumin nanoparticles and free curcumin in human telomerase reverse transcriptase gene expression in breast cancer. Adv Pharm Bull 2013; 3: 127-130
    PubMedSuche in Google Scholar
  • 40 Yallapu MM, Jaggi M, Chauhan SC. β-Cyclodextrin-curcumin self-assembly enhances curcumin delivery in prostate cancer cells. Colloids and Surfaces B: Biointerfaces 2010; 79: 113-125
    CrossrefPubMedSuche in Google Scholar
  • 41 Cogulu O, Biray C, Gunduz C. et al. Effects of Manisa propolis on telomerase activity in leukemia cells obtained from the bone marrow of leukemia patients. International Journal of Food Sciences and Nutrition 2009; 60: 601-605
    CrossrefPubMedSuche in Google Scholar
  • 42 Bozic I, Reiter JG, Allen B. et al. Evolutionary dynamics of cancer in response to targeted combination therapy. Elife 2013; 2: e00747
    CrossrefPubMedSuche in Google Scholar
  • 43 Farajzadeh R, Pilehvar-Soltanahmadi Y, Dadashpour M. et al. Nano-encapsulated metformin-curcumin in PLGA/PEG inhibits synergistically growth and hTERT gene expression in human breast cancer cells. Artificial Cells, Nanomedicine, and Biotechnology 2017; 1-9
    PubMedSuche in Google Scholar
  • 44 Maasomi ZJ, Soltanahmadi YP, Dadashpour M. et al. Synergistic anticancer effects of silibinin and chrysin in T47D breast cancer cells. Asian Pacific Journal Of Cancer Prevention: APJCP 2017; 18: 1283
    PubMedSuche in Google Scholar
  • 45 Chen H, Landen CN, Li Y. et al. Epigallocatechin gallate and sulforaphane combination treatment induce apoptosis in paclitaxel-resistant ovarian cancer cells through hTERT and Bcl-2 down-regulation. Experimental Cell Research 2013; 319: 697-706
    CrossrefPubMedSuche in Google Scholar
  • 46 Amirsaadat S, Pilehvar-Soltanahmadi Y, Zarghami F. et al. Silibinin-loaded magnetic nanoparticles inhibit hTERT gene expression and proliferation of lung cancer cells. Artificial cells, nanomedicine, and biotechnology 2017; 45: 1649-1656
    CrossrefPubMedSuche in Google Scholar
  • 47 Danafar H. Applications of copolymeric nanoparticles in drug delivery systems. Drug Research 2016; 66: 506-519
    Thieme ConnectPubMedSuche in Google Scholar