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Horm Metab Res 2016; 48(06): 413-415
DOI: 10.1055/s-0042-105151
Immediate Interest
© Georg Thieme Verlag KG Stuttgart · New York

Expression of Estrogen Receptors Alpha (ER-α), Beta (ER-β), and G Protein-Coupled Receptor 30 (GPR30) in Testicular Tissue of Men with Klinefelter Syndrome

R. L. Bernardino
1   Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal
,
M. G. Alves
2   CICS – UBI – Health Sciences Research Centre, University of Beira Interior, Covilhã, Portugal
,
J. Silva
3   Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal
,
A. Barros
3   Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal
4   Department of Genetics, Faculty of Medicine, University of Porto, Portugal
,
L. Ferraz
5   Department of Urology, Hospital Center of Vila Nova de Gaia, Vila Nova de Gaia, Portugal
,
M. Sousa
1   Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal
3   Centre for Reproductive Genetics Prof. Alberto Barros, Porto, Portugal
,
R. Sá
1   Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal
,
P. F. Oliveira
1   Department of Microscopy, Laboratory of Cell Biology, Institute of Biomedical Sciences Abel Salazar (ICBAS) and Multidisciplinary Unit for Biomedical Research (UMIB), University of Porto, Portugal
6   Institute of Health Research and Innovation (I3S), University of Porto, Porto, Portugal
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Further Information

Publication History

received 18 November 2015

accepted 03 March 2016

Publication Date:
12 May 2016 (online)

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Abstract

Men with Klinefelter syndrome (KS) present severe hormonal dysregulation, particularly elevated serum estradiol concentration. Estrogens act through specific receptors and regulate testes development and spermatogenesis. Herein, we evaluated GPR30, ERα, and ERβ mRNA expression in testis of KS men and men with 46XY karyotype by reverse transcriptase and quantitative PCR. ERβ transcripts are the most abundant in testicular tissue of 46XY men. Notably, testicular GPR30 transcription in KS men was approximately 12 times higher. Since GPR30 is essential to mediate estrogen effects over steroidogenesis, our data illustrate that GPR30 may underpin the testicular alterations observed in KS men.

Key words

estrogen receptors - Klinefelter syndrome - spermatogenesis - fertility
 

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