The Plasmatic Atmosphere of Blood Platelets. Evidence that only Fibrinogen, AcG, and Activated Hageman Factor are Present on the Surface of Platelets.^[*]

Summary

Washed platelet suspensions contain active SF (surface factor or activation product), when prepared from normal (N) subjects or from patients deficient in factors Vili, IX or X, but not from a Hageman-deficient. Factor V-deficient plasmas were normalized by platelets from N, XII- and other subjects. Allowing for (a) the platelet prothromboplastic phospholipids, and (b) the above SF and AcG (V) effects, there was no evidence of any significant factors Vili, IX or X in the platelet plasmatic atmosphere. Since XII-platelets showed no true correction of plasmas deficient in Vili, IX or X, the minor “improvement” of test results in these (and N) plasmas on adding N, Vili-, IX- or X-platelets can be explained as a nonspecific surface factor effect. XII-platelets when incubated with normal plasma were normalized by adsorbing Hageman factor (XII) on their surface.

^* This study was aided by USPHS Research Grant No. HE-01510-10, and NIH Institutional Research Grant HE-06350-02 for Thrombosis Research.

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