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Thromb Haemost 1966; 16(01/02): 198-206
DOI: 10.1055/s-0038-1655637
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

A Simple Method for Preparing Fibrinogen[*]

W Straughn III
1   Department of Pathology and Biochemistry, University of North Carolina, Chapel Hill, North Carolina
,
R. H Wagner   **
1   Department of Pathology and Biochemistry, University of North Carolina, Chapel Hill, North Carolina
› Author Affiliations
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Publication History

Publication Date:
26 June 2018 (online)

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Summary

A simple new procedure is reported for the isolation of canine, bovine, porcine, and human fibrinogen. Two molar β-alanine is used to precipitate fibrinogen from barium sulfate adsorbed plasma. The procedure is characterized by dependability and high yields. The material is 95% to 98% clottable protein but still contains impurities such as plasminogen and fibrin-stabilizing factor. Plasminogen may be removed by adsorption with charcoal. The fibrinogen preparations exhibit marked stability to freezing, lyophilization, and dialysis. Epsilon-amino-n-caproic acid and gamma-aminobutyric acid which were also studied have the property of precipitating proteins from plasma but lack the specificity for fibrinogen found with β-alanine.

* This investigation was supported by USPHS grants HE-01648 and HE-06350 from the National Institutes of Health.


** This investigator was supported by USPHS research career program award (K3-HE-1147).


 

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