Purification of Human Plasminogen

Summary

Highly purified Kline-type plasminogen was obtained from Fraction III of human blood plasma by acid-salt extraction and precipitation procedures. Fraction III was washed with 1 M NaCl at pH 2 to remove interfering substances. Plasminogen was then extracted with 0.2 M NaCl at pH 2 and was further purified by precipitating it at pH 5.8 in the absence and in the presence of 0.1 M lysine. Highly purified material, although in varying yield, was obtained from all 3 lots of Fraction III (dried powder) and the 1 lot of Fraction III (frozen paste) used.

• References

• 1 Kline D. L, Fishman J. B. Improved procedure for the isolation of human plasminogen. J. biol. Chem 236: 3232 1961;
• 2 Roberts P. S. The esterase activities of human plasmin during purification and subsequent activation by streptokinase or glycerol. J. biol. Chem 235: 2262 1960;
• 3 Personal Communication, Dr. James H. Pert, Research Director, American Red Cross Blood Program.
• 4 Roberts P. S. Measurement of the rate of plasmin action on synthetic substrates. J. biol. Chem 232: 285 1958;
• 5 Waddell W. J. A simple ultraviolet spectrophotometry method for the determination of protein. J. Lab. clin. Med 48: 311 1956;