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Thromb Haemost 1968; 19(01/02): 204-212
DOI: 10.1055/s-0038-1651197
Originalarbeiten – Original Articles – Travaux Originaux
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Transformation of Autoprothrombin III to Autoprothrombin C in Sodium Citrate Solution[*]

R Kipfer
1   Department of Physiology and Pharmacology, Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
,
W. H Seegers
1   Department of Physiology and Pharmacology, Wayne State University, School of Medicine, Detroit, Michigan, U.S.A.
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Publication History

Publication Date:
27 June 2018 (online)

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Summary

All reactions studied occurred in 25% sodium citrate solution. The conversion of prethrombin to thrombin with autoprothrombin C was retarded by 3,4,4’-triaminodi-phenyl sulfone. The compound functioned as a competitive inhibitor. Purified autoprothrombin III converted to autoprothrombin C more rapidly when autoprothrombin C was added at zero time. Soybean trypsin inhibitor, which neutralizes autopro-thrombin C activity, blocked the conversion of autoprothrombin III to autoprothrom-bin C, and 3,4,4’-triaminodiphenylsulf one inhibited the development of autoprothrombin C activity. The activation of prothrombin in 25% sodium citrate solution consists of three main events; namely, 1. the dissociation of prothrombin into subunits, 2. the formation of autoprothrombin C, and 3. the formation of thrombin.

* This investigation was supported by research grant HE-03424-10 and 5-FI-GM-30, 228-02 from the National Institutes of Health, U. S. Public Health Service.


 

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