Download PDF
Thromb Haemost 1968; 19(01/02): 161-168
DOI: 10.1055/s-0038-1651192
Originalarbeiten – Original Articles – Travaux Originaux
Schattauer GmbH

A Method to Correct for the Continuing Activation during the Second Stage in a Two-Stage Assay

Exemplified by Urokinase Activation of Plasminogen Determined by the Lysis Time Method
W Berg
1   Medical Department II, Sahlgrenska Hospital, University of Göteborg, Sweden
› Author Affiliations
Further Information

Publication History

Publication Date:
27 June 2018 (online)

    Permissions and Reprints

Summary

In coagulation and fibrinolysis, kinetic data are difficult to obtain with ordinary quantitative methods because no simple means are available to stop the reaction before the determination of the activity formed.

This work describes how to calculate, from the data recorded, the amount of activity at the moment the determination is started.

A formula is given for a zero, a first and a second-order reaction.

The method is exemplified by urokinase activation of plasminogen into plasmin. The determination of the plasmin activity is done by means of the lysis time method.

 

  • References

  • 1 Alkjaersig N, Fletcher A.P, Sherry S. The activation of human plasminogen. J. biol. Chem. 1958; 233: 86
    PubMedGoogle Scholar
  • 2 Berg W. The urokinase activation of plasminogen and inactivation of the plasmin formed. A kinetic study. Thrombos. Diathes. haemorrh. (Stuttg.) 1967; 19: 161
    PubMedGoogle Scholar
  • 3 Berg W, Korsan-Bengtsen K, Ygge J. Plasminogen assay by means of the lysis time method. Thrombos. Diathes. haemorrh. (Stuttg.) 1965; 14: 127
    PubMedGoogle Scholar
  • 4 Berg W, Korsan-Bengtsen K. Separation of human fibrinogen and plasminogen by means of gel filtration. Thrombos. Diathes. haemorrh. (Stuttg.) 1963; 9: 151
    PubMedGoogle Scholar
  • 5 Berg W, Korsan-Bengtsen K, Ygge J. Preparation of human plasminogen suitable as substrate for quantitative determination of plasminogen activators. Thrombos. Diathes. haemorrh. (Stuttg.) 1966; 15: 511
    PubMedGoogle Scholar
  • 6 Berg W, Korsan-Bengtsen K, Ygge J. Human and bovine plasminogen-free thrombin, purified by means of gel filtration and ion exchange chromatography. Thrombos. Diathes. haemorrh. (Stuttg.) 1966; 15: 501
    PubMedGoogle Scholar
  • 7 Berg W, Korsan-Bengtsen K, Ygge J. Theoretical basis and standardization of the one-stage lysis time method for determination of urokinase. Thrombos. Diathes. haemorrh. (Stuttg.) 1967; 19: 169
    PubMedGoogle Scholar
  • 8 Derechin M. The assay of human plasminogen with casein as substrate. Biochem. J. 1961; 78: 443
    CrossrefPubMedGoogle Scholar
  • 9 Kjeldgaard N.O, Ploug J. Urokinase an activator of plasminogen from human urine. II Mechanism of plasminogen activation. Biochim. biophys. Acta (Amst.) 1957; 24: 283
    PubMedGoogle Scholar
  • 10 Mackay M. Fractionation and standardization of plasma fibrinolytic components. Brit. med. Bull. 1964; 20: 189
    CrossrefPubMedGoogle Scholar