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Thromb Haemost 1968; 19(01/02): 012-017
DOI: 10.1055/s-0038-1650204
Originalarbeiten – Original Articles – Travaux Originaux
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Effects of Dimethyl Sulphoxide and of Vitamin A on Human Platelets

M. G Davey   *
1   Theodor Kocher Institute, University of Berne
,
E. F Lüscher
1   Theodor Kocher Institute, University of Berne
› Author AffiliationsThis work was supported by the National Heart Foundation of Australia and the Kommission zur Förderung der Eiweißforschung an der Universität Bern. Enzyme measurements were kindly carried out by Dr. A. M. Siegel.
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Publication History

Publication Date:
27 June 2018 (online)

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Summary

Dimethyl sulphoxide (DMSO) causes some release of amino acids, adenine nucleotides, and acid phosphatase and β-glucuronidase activities from washed human platelets. Addition of vitamin A with DMSO greatly enhances the effect; but vitamin A in ethanol added to platelets fails to release amino acids and nucleotides. Release appears to be due to a change in permeability of the platelet membrane, not to the discharge of contents of intracellular structures. Lipid peroxidation of membrane phospholipids, or a similar reaction, may be involved. Thrombin causes a different type of alteration in platelet permeability.

The results suggest that DMSO should not be used as a preservative for platelets. They may have some bearing on the mechanisms of toxicity of DMSO and vitamin A.

* Present address: Red Cross Blood Transfusion Service, 290, Wellington Street, Perth, W. A., Australia.


 

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