Thromb Haemost 1966; 15(03/04): 338-342
DOI: 10.1055/s-0038-1649435
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

The Separation of AHF from Fibrinogen. IV[*]

S van Creveld
,
I. A Mochtar
,
C. N Pascha
**   Hemofilia Clinic, Huizen (N. H.), the Netherlands.
› Author Affiliations
Further Information

Publication History

Publication Date:
27 June 2018 (online)

Summary

The anti-hemophilic factor (AHF, factor VIII) has been separated from fibrinogen by means of continous flow electrophoresis. The starting material was Cohn’s fraction I, prepared from normal human resinplasma. These fibrinogen-free products still contained proteins without AHF-activity. Part of these contaminating proteins could be extracted from fraction I by means of a 1 molar solution of epsilon amino caproic acid. The residue, principally consisting of fibrinogen and AHF, was dissolved in a triethylamine buffer and subjected to continuous flow electrophoresis. The resulting AHF-preparations were free from fibrinogen. By immuno-electrophoresis no proteins without AHF-activity could be demonstrated in these preparations.

* This investigation was supported by the Netherlands Organization for Pure Scientific Research (Z.W. 0.).