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Thromb Haemost 2001; 85(02): 309-313
DOI: 10.1055/s-0037-1615685
Review Article
Schattauer GmbH

Time and Dose Dependent Augmentation of Inhibitory Effects of Abciximab by Aspirin

David J. Schneider
1   University of Vermont, Burlington, VT, USA
,
Patricia Q. Baumann
1   University of Vermont, Burlington, VT, USA
,
Michael B. Holmes
1   University of Vermont, Burlington, VT, USA
,
Douglas Taatjes
*   Departments of Medicine and Pathology, University of Vermont, Burlington, VT, USA
,
Burton E. Sobel
1   University of Vermont, Burlington, VT, USA
› Author Affiliations
Further Information

Publication History

Received 30 May 2000

Accepted after resubmission 31 August 2000

Publication Date:
08 December 2017 (online)

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Summary

Aspirin and abciximab independently decrease the incidence of cardiac events. To identify potential interactions, antiplatelet effects of abciximab were characterized in blood from healthy subjects given aspirin. Platelet activation was determined in whole blood with and without abciximab (2 μg/ml) added in vitro. Flow cytometry was used to quantify fibrinogen binding (glycoprotein IIb-IIIa activation). Binding of fluorochrome-labeled and 125I-labeled abciximab was determined before and after exposure to aspirin. In blood from subjects given aspirin for 5 days, abciximab-induced inhibition of the capacity to bind fibrinogen in response to 1 μM ADP was greater when the daily dose had been 325 mg compared with 81 mg (% inhibition: no aspirin 53 ± 6; 81 mg daily 62 ± 5; 325 mg daily 69 ± 6). The effect of 5 daily doses of aspirin was greater than that of one. Larger single doses elicited larger effects (% inhibition 2 h after 325 mg 59 ± 6; 2 h after 650 mg 78 ± 5). Neither salicylsalicylic acid nor naproxen sodium potentiated the effect of abciximab. Exposure of platelets to 14Cacetylsalicylic acid led to acetylation of glycoprotein IIb and IIIa. Binding of 125I-abciximab to platelets was increased after 30 and 60 min. Acetylation of glycoprotein IIb-IIIa by aspirin augments inhibitory effects of abciximab in a dose- and time-dependent manner by increasing binding of abciximab to platelets.

Keywords

Platelets - glycoproteins - aspirin - platelet aggregation inhibitors

* University of Vermont, Burlington, VT, USA


 

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