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Thromb Haemost 2000; 83(02): 248-252
DOI: 10.1055/s-0037-1613795
Rapid Communication
Schattauer GmbH

An Automated Heteroduplex Assay for the PlA Polymorphism of Glycoprotein IIb/IIIa, Multiplexed with Two Prothrombotic Genetic Markers[*]

Fiona O’Connor
1   From the Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland
,
Desmond J. Fitzgerald
1   From the Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland
,
Ronan P. Murphy
1   From the Department of Clinical Pharmacology, Royal College of Surgeons in Ireland, Dublin, Ireland
› Author Affiliations
Further Information

Publication History

Received 08 June 1999

Accepted after revision 15 October 1999

Publication Date:
27 December 2017 (online)

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Summary

Screening for the PlA polymorphism has been made faster and simpler with the advent of heteroduplex technology. Simultaneous screening for three common prothrombotic polymorphisms PlA, factor V Leiden, and MTHFR(C677T) has been achieved with multiplex heteroduplex analysis. We describe a quick and simple method for PlA heteroduplex probe production. The probe was multiplexed with heteroduplex probes for MTHFR(C677T) and factor V Leiden polymorphisms in a one tube assay, allowing rapid automated genotyping of all three. This automated multiplex assay was applied to a cohort of 165 patients and showed excellent correlation with gel-based assays, both PAGE and RFLP. This approach will facilitate the analysis of multiple polymorphisms in complex disease in large populations.

Keywords

PlA - factor V Leiden - thermolabile MTHFR polymorphisms - automated heteroduplex assay

* Supported by grants from the Wellcome Trust, the Higher Education Authority (HEA) and the Health Research Board (HRB) of Ireland. Dr. Murphy was a HRB Postdoctoral Fellow during the performance of this work.


 

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