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Thromb Haemost 2003; 89(02): 365-373
DOI: 10.1055/s-0037-1613454
Wound Healing and Inflammation/Infection
Schattauer GmbH

Effect of clotting factors concentrates on lymphocyte and neutrophil function in vitro

Relevance of soluble HLA class I, soluble Fas ligand and transforming growth factor β1
Massimo Ghio
1   Department of Internal Medicine (DIMI), University of Genoa
,
Paola Contini
1   Department of Internal Medicine (DIMI), University of Genoa
,
Luciano Ottonello
1   Department of Internal Medicine (DIMI), University of Genoa
,
Nicoletta Arduino
1   Department of Internal Medicine (DIMI), University of Genoa
,
Alessandro Gringeri
2   A. Bianchi Bonomi Hemophilia and Thrombosis Centre, IRCCS Maggiore Hospital and University of Milan, Italy
,
Francesco Indiveri
1   Department of Internal Medicine (DIMI), University of Genoa
,
Franco Dallegri
1   Department of Internal Medicine (DIMI), University of Genoa
,
Francesco Puppo
1   Department of Internal Medicine (DIMI), University of Genoa
› Author Affiliations
Further Information

Publication History

Received 16 October 2002

Accepted after revision 25 November 2002

Publication Date:
07 December 2017 (online)

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Summary

Immunological abnormalities have been reported in haemophiliacs. Although infections with HIV, hepatitis and other viruses may contribute to these abnormalities, immune defects are detectable also in HIV seronegative haemophiliacs. It is likely that chronic exposure to extraneous proteins in clotting factor concentrates (CFCs) may play a role in immunomodulation, but the underlying mechanisms remain unclear. The results of the present paper show that: a) soluble HLA class I (sHLA-I), soluble Fas-ligand (sFas-L) and transforming growth factor beta 1 (TGF-β1) are detectable in plasma derived but not in recombinant CFCs; b) the level of sHLA-I and sFas-L is proportional to the grade of CFCs purity whereas TGF-β1 showed very variable levels; c) soluble molecules detected in CFCs exert immunomodulatory effects in vitro like apoptosis induction in Jurkat cells and inhibition of mixed lymphocyte reaction response, antigen-specific lymphocyte cytotoxic activity and neutrophil chemotaxis.

Keywords

Clotting factors concentrates - soluble HLA class I - soluble Fas ligand - transforming growth factor β1
 

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