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DOI: 10.1055/s-0034-1382838
Triterpenoid Saponins from the Roots of Clematis argentilucida and Their Cytotoxic Activity
Publication History
received 24 March 2014
revised 09 June 2014
accepted 13 June 2014
Publication Date:
16 July 2014 (online)
![](https://www.thieme-connect.de/media/plantamedica/201411/lookinside/thumbnails/10.1055-s-0034-1382838-1.jpg)
Abstract
The reinvestigation of the n-BuOH extract of the roots of Clematis argentilucida led to the isolation of four new oleanane-type triterpenoid saponins, 1–4, four known saponins, 5–8, first isolated from the species, together with ten saponins, 9−18, reported in the preceding papers. The structures of saponins 1–8 were elucidated by extensive spectroscopic analysis and chemical evidences. The cytotoxicity of all the saponins were evaluated against human tumor HL-60, HepG-2, and SGC-7901 cell lines. The monodesmosidic saponins 4, 7, 8, and 14–18 exhibited cytotoxic activity against the three cell lines with IC50 values in the range of 0.87–19.48 µM.
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References
- 1 Jiang N, Guan KY, Wang ZL. Species diversity and geographical distribution of Clematis (Ranunculaceae) in Yunnan. Acta Botanica Yunnanica 2007; 29: 145-154
- 2 Chinese Pharmacopoeia Comittee. Chinese Pharmacopoeia, Vol. 1. Beijing: Chemical Industry Press; 2010: 234-235
- 3 Zhao M, Tang HF, Qiu F, Tian XR, Ding Y, Wang XY, Zhou XM. Triterpenoid saponins from Clematis argentilucida . Biochem Syst Ecol 2012; 40: 49-52
- 4 Zhao M, Ma N, Hai WL, Tian XR, Tang HF, Qiu F. Chemical constituents of Clematis argentilucida . Zhongnan Yaoxue 2011; 5: 339-342
- 5 Hai WL, Cheng H, Zhao M, Wang Y, Hong LJ, Tang HF, Tian XR. Two new cytotoxic triterpenoid saponins from the roots of Clematis argentilucida . Fitoterapia 2012; 83: 759-764
- 6 Dong FY, Cui GH, Zhang YH, Zhu RN, Wu XJ, Sun TT, Wang W. Clematomandshurica saponin E, a new triterpenoid saponin from Clematis mandshurica . J Asian Nat Prod Res 2010; 12: 1061-1068
- 7 Fu Q, Zan K, Zhao MB, Zhou SX, Shi SP, Jiang Y, Tu PF. Triterpene saponins from Clematis chinensis and their potential anti-inflammatory activity. J Nat Prod 2010; 73: 1234-1239
- 8 Zheng Q, Koike K, Han LK, Okuda H, Nikaido T. New biologically active triterpenoid saponins from Scabiosa tschiliensis . J Nat Prod 2004; 67: 604-613
- 9 Wang Y, Kang W, Hong LJ, Hai WL, Wang XY, Tang HF, Tian XR. Triterpenoid saponins from the root of Anemone tomentosa . J Nat Med 2013; 67: 70-77
- 10 Mimaki Y, Yokosuka A, Hamanaka M, Sakuma C, Yamori T, Sashida Y. Triterpene saponins from the roots of Clematis chinensis . J Nat Prod 2004; 67: 1511-1516
- 11 Ye WC, Zhang QW, Zhao SX, Che CT. Four new oleanane saponins from Anemone anhuiensis . Chem Pharm Bull 2001; 49: 632-634
- 12 Zhao L, Chen WM, Fang QC. Two new oleanane saponins from Anemone flaccida . Planta Med 1991; 57: 572-574
- 13 Lin YP, Chui MH, Li ZR. Two new compounds from Hemsleya penxianensis var. gulinensis . Chinese Chem Lett 2003; 14: 169-172
- 14 Tian XR, Feng JT, Tang HF, Zhao M, Li Y, Hai WL, Zhang X. New cytotoxic triterpenoid saponins from the whole plant of Clematis lasiandra Maxim. Fitoterapia 2013; 90: 233-239
- 15 Shen L, Ye YH, Wang XT, Zhu HL, Xu C, Song YC, Li H, Tan RX. Structure and total synthesis of aspernigerin: a novel cytotoxic endophyte metabolite. Chem Eur J 2006; 12: 4393-4396
- 16 Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983; 65: 53-63
- 17 Wang XY, Chen XL, Tang HF, Gao H, Tian XR, Zhang PH. Cytotoxic triterpenoid saponins from the rhizomes of Anemone taipaiensis . Planta Med 2011; 77: 1550-1554
- 18 Wang XY, Gao H, Zhang W, Li Y, Cheng G, Sun XL, Tang HF. Bioactive oleanane-type saponins from the rhizomes of Anemone taipaiensis . Bioorg Med Chem Lett 2013; 23: 5714-5720
- 19 Yokosuka A, Sano T, Hashimoto K, Sakagami H, Mimaki Y. Triterpene glycosides from the whole plant of Anemone hupehensis var. japonica and their cytotoxic activity. Chem Pharm Bull 2009; 57: 1425-1430
- 20 Bang SC, Lee JH, Song GY, Kim DH, Yoon MY, Ahn BZ. Antitumor activity of Pulsatilla koreana saponins and their structure-activity relationship. Chem Pharm Bull 2005; 53: 1451-1454
- 21 Man S, Gao W, Zhang Y, Huang L, Liu C. Chemical study and medical application of saponins as anti-cancer agents. Fitoterapia 2010; 81: 703-714