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Planta Med 2012; 78(7): 740-746
DOI: 10.1055/s-0031-1298373
Analytical Studies
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Metabolic Profiling Assisted Quality Assessment of Rhodiola rosea Extracts by High-Performance Liquid Chromatography

Zhanguo Wang1 , Huiling Hu2 , Fang Chen1 , Liang Zou3 , Mingfu Yang4 , Anqi Wang5 , James E. Foulsham6 , Ke Lan5
  • 1College of Life Sciences, Sichuan University, Chengdu, Sichuan, China
  • 2College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu, Sichuan, China
  • 3College of Biological Industry, Chengdu University, Chengdu, Sichuan, China
  • 4Chengdu Wagott Pharmaceutical Co. Ltd., Chengdu, Sichuan, China
  • 5Key Laboratory of Drug Targeting and Drug Delivery Systems, Ministry of Education, West China School of Pharmacy, Sichuan University, Sichuan, China
  • 6Department of Pediatric Orthopedic Surgery and Sports Medicine, Seattle Children's Hospital & School of Medicine, Clinical Faculty, University of Washington, Seattle, WA, USA
Further Information

Publication History

received Dec. 5, 2011 revised February 16, 2012

accepted February 22, 2012

Publication Date:
22 March 2012 (online)

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Abstract

In this work, fast and sensitive high-performance liquid chromatography (HPLC) coupled with multivariate analysis was utilized to assist the quality assessment of Rhodiola rosea extracts (RREs). 131 peaks were separated and detected in RREs on a fused-core C18 column. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) of the chromatographic data demonstrated that 10 batches of RREs could be well-differentiated and categorized into three groups which were closely related to the origins of RREs. Partial least square-discriminant analysis (PLS-DA) showed that the quality differentiation might be explained by at least 6 components, in which rosavin was characterized by an external reference, rosiridine was identified by liquid chromatography–mass spectrometry (LC-MS), and the mass spectra of the others were provided. The observation that the level of rosavin was more relevant to the multivariate chromatographic data than the ones of salidroside and tyrosol, the other two components commonly used to standardize RREs, was confirmed by the PLS prediction models. Results of the present study not only indicated that rosavin was a rational marker to represent the quality of RREs, but also demonstrated the power of HPLC-based metabolic profiling in the quality assessment of herbal extracts.

Key words

metabolic profiling - Rhodiola rosea L. extracts - Crassulaceae family - quality assessment - high-performance liquid chromatography - fused-core column

Supporting Information

References

  • 1 Xie P, Chen S, Liang Y Z, Wang X, Tian R, Upton R. Chromatographic fingerprint analysis–a rational approach for quality assessment of traditional Chinese herbal medicine.  J Chromatogr A. 2006;  1112 171-180
    Google Scholar
  • 2 Chen Y, Zhu S B, Xie M Y, Nie S P, Liu W, Li C, Gong X F, Wang Y X. Quality control and original discrimination of Ganoderma lucidum based on high-performance liquid chromatographic fingerprints and combined chemometrics methods.  Anal Chim Acta. 2008;  623 146-156
    Google Scholar
  • 3 Jin X F, Lu Y H, Wei D Z, Wang Z T. Chemical fingerprint and quantitative analysis of Salvia plebeia R.Br. by high-performance liquid chromatography.  J Pharm Biomed Anal. 2008;  48 100-104
    Google Scholar
  • 4 Li W, Deng Y, Dai R, Yu Y, Saeed M K, Li L, Meng W, Zhang X. Chromatographic fingerprint analysis of Cephalotaxus sinensis from various sources by high-performance liquid chromatography-diodearray detection-electrospray ionization-tandem mass spectrometry.  J Pharm Biomed Anal. 2007;  45 38-46
    Google Scholar
  • 5 Alaerts G, Matthijs N, Smeyers-Verbeke J, Vander Heyden Y. Chromatographic fingerprint development for herbal extracts: a screening and optimization methodology on monolithic columns.  J Chromatogr A. 2007;  1172 1-8
    Google Scholar
  • 6 Chan E C, Yap S L, Lau A J, Leow P C, Toh D F, Koh H L. Ultra-performance liquid chromatography/time-of-flight mass spectrometry based metabolomics of raw and steamed Panax notoginseng.  Rapid Commun Mass Spectrom. 2007;  21 519-528
    Google Scholar
  • 7 Liang Y Z, Xie P S, Chan K. Chromatographic fingerprinting and metabolomics for quality control of TCM.  Comb Chem High Throughput Screen. 2010;  13 943-953
    Google Scholar
  • 8 Liang X, Zhang L, Zhang X, Dai W, Li H, Hu L, Liu H, Su J, Zhang W. Qualitative and quantitative analysis of traditional Chinese medicine Niu Huang Jie Du Pill using ultra performance liquid chromatography coupled with tunable UV detector and rapid resolution liquid chromatography coupled with time-of-flight tandem mass spectrometry.  J Pharm Biomed Anal. 2010;  51 565-571
    Google Scholar
  • 9 Wei S Y, Xu C J, Mok D K, Cao H, Lau T Y, Chau F T. Analytical comparison of different parts of Radix Angelicae Sinensis by gas chromatography coupled with mass spectrometry.  J Chromatogr A. 2008;  1187 232-238
    Google Scholar
  • 10 Qiu Y, Lu X, Pang T, Ma C, Li X, Xu G. Determination of radix ginseng volatile oils at different ages by comprehensive two-dimensional gas chromatography/time-of-flight mass spectrometry.  J Sep Sci. 2008;  31 3451-3457
    Google Scholar
  • 11 Tan S N, Yong J W, Teo C C, Ge L, Chan Y W, Hew C S. Determination of metabolites in Uncaria sinensis by HPLC and GC-MS after green solvent microwave-assisted extraction.  Talanta. 2011;  83 891-898
    Google Scholar
  • 12 Lan K, Zhang Y, Yang J, Xu L. Simple quality assessment approach for herbal extracts using high performance liquid chromatography-UV based metabolomics platform.  J Chromatogr A. 2010;  1217 1414-1418
    Google Scholar
  • 13 Panossian A, Wikman G, Sarris J. Rosenroot (Rhodiola rosea): traditional use, chemical composition, pharmacology and clinical efficacy.  Phytomedicine. 2010;  17 481-493
    Google Scholar
  • 14 Blomkvist J, Taube A, Larhammar D. Perspective on Roseroot (Rhodiola rosea) studies.  Planta Med. 2009;  75 1187-1190
    Google Scholar
  • 15 Ssaratikov A S, Krasnov E A, Chnikina L A, Duvidson L M, Sotova M I, Marina T F, Nechoda M F, Axenova R A, Tscherdinzeff S G. [Rhodiolosid, a new glycoside from Rhodiola rosea and its pharmacological properties].  Pharmazie. 1968;  23 392-395
    Google Scholar
  • 16 Khnykina L A, Peshekhonova R I, Gol'tsev V D. [Photometric determination of rhodioloside in extracts of Rhodiola rosea L].  Farmatsiia. 1973;  22 24-27
    Google Scholar
  • 17 Yousef G G, Grace M H, Cheng D M, Belolipov I V, Raskin I, Lila M A. Comparative phytochemical characterization of three Rhodiola species.  Phytochemistry. 2006;  67 2380-2391
    Google Scholar
  • 18 Ganzera M, Yayla Y, Khan I A. Analysis of the marker compounds of Rhodiola rosea L. (golden root) by reversed phase high performance liquid chromatography.  Chem Pharm Bull (Tokyo). 2001;  49 465-467
    Google Scholar
  • 19 Ma Y C, Wang X Q, Hou F F, Ma J, Luo M, Lu S, Jin P, Terevsky N, Chen A, Xu I, Pate A V, Gorecki D. Rapid resolution liquid chromatography (RRLC) analysis for quality control of Rhodiola rosea roots and commercial standardized products.  Nat Prod Commun. 2011;  6 645-650
    Google Scholar
  • 20 Ma Y C, Wang X Q, Hou F, Ma J, Luo M, Lu S, Jin P, Chen A, Xu I, Patel A V, Gorecki D. Simultaneous quantification of polyherbal formulations containing Rhodiola rosea L. and Eleutherococcus senticosus Maxim. using rapid resolution liquid chromatography (RRLC).  J Pharm Biomed Anal. 2011;  55 908-915
    Google Scholar
  • 21 Avula B, Wang Y H, Ali Z, Smillie T J, Filion V, Cuerrier A, Arnason J T, Khan I A. RP-HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC-ESI-TOF.  Biomed Chromatogr. 2009;  23 865-872
    Google Scholar
  • 22 Avula B, Wang Y H, Ali Z, Smillie T J, Filion V, Cuerrier A, Arnason J T, Khan I A. Erratum: RP-HPLC determination of phenylalkanoids and monoterpenoids in Rhodiola rosea and identification by LC-ESI-TOF.  Biomed Chromatogr. 2010;  24 682
    Google Scholar
  • 23 Ioset K N, Nyberg N T, Van Diermen D, Malnoe P, Hostettmann K, Shikov A N, Jaroszewski J W. Metabolic profiling of Rhodiola rosea rhizomes by (1)H NMR spectroscopy.  Phytochem Anal. 2010;  22 158-165
    Google Scholar
  • 24 Sangster T, Major H, Plumb R, Wilson A J, Wilson I D. A pragmatic and readily implemented quality control strategy for HPLC-MS and GC-MS-based metabonomic analysis.  Analyst. 2006;  131 1075-1078
    Google Scholar
  • 25 Peng Y, Luo J, Lu Q, Chen X, Xie Y, Chen L, Yang W, Du S. HPLC analysis, semi-preparative HPLC preparation and identification of three impurities in salidroside bulk drug.  J Pharm Biomed Anal. 2009;  49 828-832
    Google Scholar
  • 26 van den Berg R A, Hoefsloot H C, Westerhuis J A, Smilde A K, van der Werf M J. Centering, scaling, and transformations: improving the biological information content of metabolomics data.  BMC Genomics. 2006;  7 142
    Google Scholar
  • 27 Zou L, Wang Z, Hu H, Yang M, Bao X, Wang A. Rapid determination of the salidroside, tyrosol and rosavin in Rhodiola rosea extract by HPLC using a fused-core column.  Chin Tradit Herb Drugs. 2011;  42 134-1337
    Google Scholar
  • 28 Rostagno M A, Manchon N, D'Arrigo M, Guillamon E, Villares A, Garcia-Lafuente A, Ramos A, Martinez J A. Fast and simultaneous determination of phenolic compounds and caffeine in teas, mate, instant coffee, soft drink and energetic drink by high-performance liquid chromatography using a fused-core column.  Anal Chim Acta. 2011;  685 204-211
    Google Scholar
  • 29 Manchon N, D'Arrigo M, Garcia-Lafuente A, Guillamon E, Villares A, Ramos A, Martinez J A, Rostagno M A. Fast analysis of isoflavones by high-performance liquid chromatography using a column packed with fused-core particles.  Talanta. 2010;  82 1986-1994
    Google Scholar
  • 30 Abrahim A, Al-Sayah M, Skrdla P, Bereznitski Y, Chen Y, Wu N. Practical comparison of 2.7 microm fused-core silica particles and porous sub-2 microm particles for fast separations in pharmaceutical process development.  J Pharm Biomed Anal. 2010;  51 131-137
    Google Scholar
  • 31 Yang P, Litwinski G R, Pursch M, McCabe T, Kuppannan K. Separation of natural product using columns packed with Fused-Core particles.  J Sep Sci. 2009;  32 1816-1822
    Google Scholar

Prof. Fang Chen, Ph.D.

College of Life Sciences
Sichuan University No. 24 South Section 1

Yihuan Road, Chengdu

Sichuan 610065

China

Email: chenfang@scu.edu.cn

Dr. Ke Lan, Ph.D.

Key Laboratory of Drug Targeting and Drug Delivery Systems
Ministry of Education
West China School of Pharmacy
Sichuan University No. 17

the 3rd section of Reminnan Road, Chengdu

Sichuan 610041

China

Phone: +86 28 85 50 37 22

Fax: +86 28 85 50 37 22

Email: lanwoco@scu.edu.cn