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DOI: 10.1055/s-0031-1271679
© Georg Thieme Verlag KG Stuttgart · New York
Lifelong Sedentary Behaviour and Femur Structure
Publication History
accepted after revision December 23, 2010
Publication Date:
04 March 2011 (online)
![](https://www.thieme-connect.de/media/sportsmed/201105/lookinside/thumbnails/10.1055-s-0031-1271679-1.jpg)
Abstract
The aim of the present study was to analyze the lifelong differences of femur structure in sedentary and physically active animal models. Thirty male C57BL/6 mice, 2 months old, were either: i) housed in cages with running wheel (AA; n=10), ii) housed in cages without running wheel (AS; n=10), iii) or sacrificed without intervention (Y; n=10). AA and AS animals were sacrificed after 23 months of housing. Right femur structure was analyzed in all animals by histomorphometry. Significant differences in several microarchitectural parameters of cancellous and cortical bone were identified between Y mice and both groups of aged mice, as well as between AA and AS groups. Lifelong physically active mice had significantly higher cancellous bone surface (Cn.BS) and trabecular number (Tb.N) and decreased trabecular separation (Tb.Sp) at both epiphyses when compared to AS animals. No differences were observed between Y and AA groups regarding osteocyte number (N.Ot) despite its significant reduction in AS animals, suggesting that age alone was not a cause for decreases in N.Ot. Our results suggest that the reduced bone quality observed in the elderly is not only a consequence of age but also of lack of physical activity since sedentary behaviour significantly aggravated the degenerative age-related bone differences.
Key words
senile osteoporosis - histomorphometry - ageing - lifelong physical activity - osteocytes
References
- 1 Felsenberg D, Silman A, Lunt M, Armbrecht G, Ismail A, Finn J, Cockerill W, Banzer D, Benevolenskaya L, Bhalla A, Bruges AJ, Cannata J, Cooper C, Dequeker J, Eastell R, Felsch B, Gowin W, Havelka S, Hoszowski K, Jajic I, Janott J, Johnell O, Kanis J, Kragl G, Lopes Vaz A, Lorenc R, Lyritis G, Masaryk P, Matthis C, Miazgowski T, Parisi G, Pols H, Poor G, Raspe H, Reid D, Reisinger W, Scheidt-Nave C, Stepan J, Todd C, Weber K, Woolf A, Yershova O, Reeve J, O'Neill T. Incidence of vertebral fracture in Europe: Results from the European Prospective Osteoporosis Study (EPOS). J Bone Miner Res. 2002; 17 716-724
- 2 Aguirre JI, Plotkin LI, Stewart SA, Weinstein RS, Parfitt AM, Manolagas SC, Bellido T. Osteocyte apoptosis is induced by weightlessness in mice and precedes osteoclast recruitment and bone loss. J Bone Miner Res. 2006; 21 605-615
- 3 Bakker A, Klein-Nulend J, Burger E. Shear stress inhibits while disuse promotes osteocyte apoptosis. Biochem Biophys Res Commun. 2004; 320 1163-1168
- 4 Blouin S, Gallois Y, Moreau MF, Basle MF, Chappard D. Disuse and orchidectomy have additional effects on bone loss in the aged male rat. Osteoporos Int. 2007; 18 85-92
- 5 Bronikowski AM, Carter PA, Morgan TJ, Garland Jr T, Ung N, Pugh TD, Weindruch R, Prolla TA. Lifelong voluntary exercise in the mouse prevents age-related alterations in gene expression in the heart. Physiol Genomics. 2003; 12 129-138
- 6 Burr DB. Muscle strength, bone mass, and age-related bone loss. J Bone Miner Res. 1997; 12 1547-1551
- 7 Butler RN, Miller RA, Perry D, Carnes BA, Williams TF, Cassel C, Brody J, Bernard MA, Partridge L, Kirkwood T, Martin GM, Olshansky SJ. New model of health promotion and disease prevention for the 21st century. BMJ. 2008; 337 a399
- 8 Chen H, Zhou X, Washimi Y, Shoumura S. Three-dimensional microstructure of the bone in a hamster model of senile osteoporosis. Bone. 2008; 43 494-500
- 9 Coleman MA, Garland Jr T, Marler CA, Newton SS, Swallow JG, Carter PA. Glucocorticoid response to forced exercise in laboratory house mice (Mus domesticus). Physiol Behav. 1998; 63 279-285
- 10 Coore HG, Denton RM, Martin BR, Randle PJ. Regulation of adipose tissue pyruvate dehydrogenase by insulin and other hormones. Biochem J. 1971; 125 115-127
- 11 Halloran BP, Ferguson VL, Simske SJ, Burghardt A, Venton LL, Majumdar S. Changes in bone structure and mass with advancing age in the male C57BL/6J mouse. J Bone Miner Res. 2002; 17 1044-1050
- 12 Harriss DJ, Atkinson G. International Journal of Sports Medicine – Ethical Standards in Sport and Exercise Science Research. Int J Sports Med. 2009; 30 701-702
- 13 Heaney RP, Abrams S, Dawson-Hughes B, Looker A, Marcus R, Matkovic V, Weaver C. Peak bone mass. Osteoporos Int. 2000; 11 985-1009
- 14 Hellekson KL. NIH releases statement on osteoporosis prevention, diagnosis, and therapy. Am Fam Physician. 2002; 66 161-162
- 15 Ingram DK. Age-related decline in physical activity: generalization to nonhumans. Med Sci Sports Exerc. 2000; 32 1623-1629
- 16 Ju YI, Sone T, Okamoto T, Fukunaga M. Jump exercise during remobilization restores integrity of the trabecular architecture after tail suspension in young rats. J Appl Physiol. 2008; 104 1594-1600
- 17 Lang T, LeBlanc A, Evans H, Lu Y, Genant H, Yu A. Cortical and trabecular bone mineral loss from the spine and hip in long-duration spaceflight. J Bone Miner Res. 2004; 19 1006-1012
- 18 Lofthus CM, Frihagen F, Meyer HE, Nordsletten L, Melhuus K, Falch JA. Epidemiology of distal forearm fractures in Oslo, Norway. Osteoporos Int. 2008; 19 781-786
- 19 Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193 265-275
- 20 Maravic M, Le Bihan C, Landais P, Fardellone P. Incidence and cost of osteoporotic fractures in France during 2001. A methodological approach by the national hospital database. Osteoporos Int. 2005; 16 1475-1480
- 21 Melton 3rd LJ. Adverse outcomes of osteoporotic fractures in the general population. J Bone Miner Res. 2003; 18 1139-1141
- 22 Moraska A, Deak T, Spencer RL, Roth D, Fleshner M. Treadmill running produces both positive and negative physiological adaptations in Sprague-Dawley rats. Am J Physiol. 2000; 279 R1321-R1329
- 23 Newhall KM, Rodnick KJ, van der Meulen MC, Carter DR, Marcus R. Effects of voluntary exercise on bone mineral content in rats. J Bone Miner Res. 1991; 6 289-296
- 24 Nguyen ND, Ahlborg HG, Center JR, Eisman JA, Nguyen TV. Residual lifetime risk of fractures in women and men. J Bone Miner Res. 2007; 22 781-788
- 25 Oeppen J, Vaupel JW. Demography. Broken limits to life expectancy. Science. 2002; 296 1029-1031
- 26 Parfitt AM, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR. Bone histomorphometry: standardization of nomenclature, symbols, and units. Report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. 1987; 2 595-610
- 27 Qiu S, Rao DS, Palnitkar S, Parfitt AM. Reduced iliac cancellous osteocyte density in patients with osteoporotic vertebral fracture. J Bone Miner Res. 2003; 18 1657-1663
- 28 Rolland Y, Abellan van Kan G, Benetos A, Blain H, Bonnefoy M, Chassagne P, Jeandel C, Laroche M, Nourhashemi F, Orcel P, Piette F, Ribot C, Ritz P, Roux C, Taillandier J, Tremollieres F, Weryha G, Vellas B. Frailty, osteoporosis and hip fracture: causes, consequences and therapeutic perspectives. J Nutr Health Aging. 2008; 12 335-346
- 29 Sambrook P, Cooper C. Osteoporosis. Lancet. 2006; 367 2010-2018
- 30 Seeman E. Bone quality: the material and structural basis of bone strength. J Bone Miner Metab. 2008; 26 1-8
- 31 Shimizu H, Sakamoto M, Sakamoto S. Bone resorption by isolated osteoclasts in living versus devitalized bone: differences in mode and extent and the effects of human recombinant tissue inhibitor of metalloproteinases. J Bone Miner Res. 1990; 5 411-418
- 32 Tatsumi S, Ishii K, Amizuka N, Li M, Kobayashi T, Kohno K, Ito M, Takeshita S, Ikeda K. Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction. Cell Metab. 2007; 5 464-475
- 33 Thomsen JS, Ebbesen EN, Mosekilde LI. Age-related differences between thinning of horizontal and vertical trabeculae in human lumbar bone as assessed by a new computerized method. Bone. 2002; 31 136-142
- 34 Turner CH. Biomechanics of bone: determinants of skeletal fragility and bone quality. Osteoporos Int. 2002; 13 97-104
- 35 van Staa TP, Dennison EM, Leufkens HG, Cooper C. Epidemiology of fractures in England and Wales. Bone. 2001; 29 517-522
- 36 Vashishth D, Verborgt O, Divine G, Schaffler MB, Fyhrie DP. Decline in osteocyte lacunar density in human cortical bone is associated with accumulation of microcracks with age. Bone. 2000; 26 375-380
- 37 Winters-Stone KM, Snow CM. Site-specific response of bone to exercise in premenopausal women. Bone. 2006; 39 1203-1209
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