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Planta Med 2011; 77(10): 1060-1067
DOI: 10.1055/s-0030-1270748
Biological Screening
Original Papers
© Georg Thieme Verlag KG Stuttgart · New York

Inhibitory Effects of Terminalia chebula Extract on Glycation and Endothelial Cell Adhesion

Hyun-Sun Lee1 , Hong Yon Cho2 , Kuen-Woo Park3 , In-Hwan Kim4 , Jong-Tak Kim5 , Mi-Hyun Nam6 , Kwang-Won Lee6
  • 1Department of Food and Nutrition, Institute of Life Resources, Korea University, Seoul, Republic of Korea
  • 2Department of Food and Biotechnology, Korea University, Chungnam, Republic of Korea
  • 3Division of Biotechnology, College of Life Sciences and Biotechnology, Korea University
  • 4Department of Food and Nutrition, Korea University, Seoul, Republic of Korea
  • 5Kocat, Inc., Bio Division, Geumcheon-gu, Seoul, Republic of Korea
  • 6Division of Food Bioscience & Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, Republic of Korea
Further Information

Publication History

received Oct. 17, 2010 revised January 10, 2011

accepted January 17, 2011

Publication Date:
09 February 2011 (online)

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Abstract

Terminalia chebula Retz. has been used in India for a long time to treat many diseases, and its extract was reported to have antidiabetic activity in vivo. In this study, T. chebula methanolic extract (TCE) containing 2.7 % chebulic acid was evaluated for its preventive effects against the formation of advanced glycation end products (AGEs) and endothelial cell dysfuction. When the effects of TCE on AGE formation and on protein crossing-linking by glycation with D-threose and lens crystallines were examined, TCE showed inhibitory activity in a dose-dependent manner, and the concentration of 1000 µg/mL presented an activity similar to that of 5 mM aminoguanidine as a positive control. Upon investigating the protective activity of TCE against AGE-induced vascular endothelium dysfunction, human umbilical vein endothelial cells (HUVEC) incubated with 100 µg/mL of AGEs had significantly enhanced reactive oxygen species (ROS) formation, whereas the treatment of T. chebula reduced AGE-induced ROS generation. The incubation of HUVEC with 100 µg/mL of AGEs caused a considerable increase in THP-1 monocytic cell adhesion, but this adhesion was reduced by the treatment of TCE. These results suggest that TCE is a potential agent for alleviating diabetic complications.

Key words

Terminalia chebula Retz. - Combretaceae - advanced glycation end products - diabetic complications - antioxidant - lens crystalline

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Prof. Kwang-Won Lee

Division of Food Bioscience & Technology
College of Life Science and Biotechnology
Korea University

5-ga, Anam-dong

Sungbuk-ku

Seoul 136-701

Republic of Korea

Phone: +82 2 32 90 30 27

Fax: +82 29 25 19 70

Email: kwangwon@korea.ac.kr