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Horm Metab Res 2010; 42(13): 950-954
DOI: 10.1055/s-0030-1267174
Animals

© Georg Thieme Verlag KG Stuttgart · New York

Combined Effects of Short-term Calorie Restriction and Exercise on Insulin Action in Normal Rats

H. Y. Jiang1 , 2 , T. Koike1 , 3 , P. Li1 , Z. H. Wang1 , Y. Kawata1 , Y. Oshida1 , 3
  • 1Department of Sports Medicine, Graduate School of Medicine, Nagoya University, Nagoya, Japan
  • 2Department of Physiology and Pathophysiology, Yanbian University Medical College, Yanji, P.R. China
  • 3Research Center of Health, Physical Fitness and Sports, Nagoya University, Nagoya, Japan
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Publication History

received 10.05.2010

accepted 31.08.2010

Publication Date:
11 October 2010 (online)

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Abstract

The present study examined the effect of combination of short-term calorie restriction (CR) and moderate exercise on insulin action in normal rats. Rats were divided randomly into 4 groups: ad libitum, sedentary (A-Sed); calorie restriction, sedentary (CR-Sed); ad libitum, exercise (A-Ex); and calorie restriction, exercise (CR-Ex). Rats in the exercise groups were run on a rodent treadmill. Rats in the CR groups were fed every alternate day. Oral glucose tolerance test (OGTT) showed improvements in both CR-Sed and A-Ex groups compared with the A-Sed group; no further improvement in glucose tolerance was observed in the CR-Ex group. In contrast, glucose infusion rates (GIRs) determined by the hyperinsulinemic-euglycemic clamp method indicated that the GIR of the CR and exercise combination was significantly better than that of the sole intervention of CR or exercise. There was no difference in the levels of fasting glucose, insulin, or high-molecular weight forms of adiponectin among the 4 groups. Protein expression of GLUT-4 in the skeletal muscle increased by exercise, but not by CR. Our findings indicate that the combination of exercise and CR may be effective in enhancing insulin sensitivity at the skeletal muscle in normal subjects.

Key words

insulin action - euglycemic clamp method - calorie restriction - exercise - glucose transporter

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Correspondence

Y. OshidaMD, PhD 

Department of Sports Medicine

Graduate School of Medicine

Nagoya University

Nagoya 464-8601

Japan

Phone: +81/52/789 3961

Fax: +81/52/789 3957

Email: oshida@htc.nagoya-u.ac.jp