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DOI: 10.1055/s-0028-1088308
© Georg Thieme Verlag KG Stuttgart · New York
Sequencing Analysis of the Medicinal Plant Stemona tuberosa and Five Related Species Existing in Thailand Based on trnH-psbA Chloroplast DNA
Publication History
Received: February 24, 2008
Revised: July 7, 2008
Accepted: August 4, 2008
Publication Date:
05 November 2008 (online)
![](https://www.thieme-connect.de/media/plantamedica/200814/lookinside/thumbnails/10.1055-s-0028-1088308-1.jpg)
Abstract
In Thailand, Stemona tuberosa Lour., S. phyllantha Gagnep., S. collinsae Craib, S. burkillii Prain, S. aphylla Craib and S. sp. are found. The identification based on morphological characters alone is difficult and can lead to confusion regarding chemical constituents and biological activities. The tuberous roots of S. tuberosa have long been used for treatment of respiratory diseases and as anthelmintics. However, accurate identification of S. tuberosa is needed to ensure efficacy. Sequence comparison indicated that these Stemona spp. could be identified from the sequence of the trnH-psbA locus. As a result of different sequence lengths, the PCR products generated from newly designed primers could be used to preliminarily group the two species, S. tuberosa and S. phyllantha, apart from others. However, these products could be further sequenced to discriminate among Stemona spp.
Key words
Stemonaceae - Stemona tuberosa - trnH-psbA - sequence analysis - chloroplast DNA
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References
- 1 Greger H. Structural relationships, distribution and biological activities of Stemona alkaloids. Planta Med. 2006; 72 99-113
- 2 Chung H S, Hon P M, Lin G, But P PH, Dong H. Antitussive activity of Stemona alkaloids from Stemona tuberosa. . Planta Med. 2003; 69 914-20
- 3 Kaltenegger E, Brem B, Mereiter K, Kalchhauser H, Kahlig H P, Hofer O. et al . Insecticidal pyrido[1,2-a]azepine alkaloids and related derivatives from Stemona species. Phytochemistry. 2003; 63 803-16
- 4 Sukrong S, Zhu S, Ruangrungsi N, Phadungcharoen T, Palanuvej C, Komatsu K. Molecular analysis of the genus Mitragyna existing in Thailand based on rDNA ITS sequences and its application to identify a narcotic species: Mitragyna speciosa. . Biol Pharm Bull. 2007; 30 1284-8
- 5 Komatsu K, Zhu S, Fushimi H, Qui T K, Cai S, Kadota S. Phylogenetic analysis based on 18S rRNA gene and matK gene sequences of Panax vietnamensis and five related species. Planta Med. 2001; 67 461-5
- 6 Techen N, Khan I A, Pan Z, Scheffler B E. The use of polymerase chain reaction (PCR) for the identification of Ephedra DNA in dietary supplements. Planta Med. 2006; 72 241-7
- 7 Kress W J, Wurdack K J, Zimmer E A, Weigt L A, Janzen D H. Use of DNA barcodes to identify flowering plants. Proc Natl Acad Sci USA. 2005; 102 8369-74
- 8 Schinnerl J, Brem B, But P P, Vajrodaya S, Hofer O, Greger H. Pyrrolo- and pyridoazepine alkaloids as chemical markers in Stemona species. Phytochemistry. 2007; 68 1417-27
Assistant Prof. Dr. Suchada Sukrong
Department of Pharmacognosy
CU-Drugs and Health Products Innovation and Promotion Center
Faculty of Pharmaceutical Sciences
Chulalongkorn University
Bangkok
Thailand 10330
Phone: +66-2-218-8364
Email: suchada.su@chula.ac.th
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