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Planta Med
DOI: 10.1055/a-2574-2730
Original Papers

A Neutral Glucan Extracted from Dried Ginger (Zingiberis Rhizoma): Preparation, Structure Characterization, and Immunomodulatory Activity

Long Sun‡
1   Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China
,
Xing Ni‡
2   Oncology Department, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu, China
,
Yulin Liu‡
1   Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China
,
Yantao Jiang
2   Oncology Department, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu, China
,
Pei-Pei Wang
1   Department of Marine Pharmacology, College of Food Science and Technology, Shanghai Ocean University, Shanghai, China
3   Marine Biomedical Science and Technology Innovation Platform of Lin-Gang Special Area, Shanghai, China
,
Jingdong Gao
2   Oncology Department, Suzhou TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Suzhou, Jiangsu, China
4   Nanjing University of Chinese Medicine, Nanjing, Jiangsu, China
› InstitutsangabenThis work was supported by the Gusu Health Talents Program Training Project in Suzhou, China (No. GSWS2020081), the Suzhou Science and Technology Plan Project (No. SKY2022059), and the Open Projects Fund of Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University (No. 2021CCG03).
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Abstract

A neutral glucan, GJ0D, was obtained from dried ginger (Zingiberis rhizoma) by enzymatic extraction and purification with column chromatography. The fine structure of GJ0D was assessed through monosaccharide composition analysis, methylation, and two-dimensional nuclear magnetic resonance. GJ0D has a relative molecular weight of 4.0 KDa and possesses a backbone consisting of 1,4-linked α-Glcp with substitution at C-6 of Glcp by T-Glcp. Immunoactivity assessment showed that GJ0D significantly upregulates the expression of IL-6, IL-1β, and TNF-α in RAW264.7 cells. The reactive oxygen species (ROS) production was also increased in RAW264.7 cells. In addition, the expression of several proteins associated with immune activation signaling pathways including TLR4, the phosphorylation of IKKβ, and NF-κB (p100 and p52) were significantly upregulated by GJ0D. These results suggest that GJ0D could promote inflammation through the TLR4/IKKβ/P100 signaling pathway, suggesting a potential application as an immunomodulating agent.

Keywords

ginger - Zingiber officinale - Zingiberaceae - structure characterization - immunomodulatory activity - glucan

Supporting Information



Publikationsverlauf

Eingereicht: 11. November 2024

Angenommen: 20. März 2025

Artikel online veröffentlicht:
14. April 2025

© 2025. Thieme. All rights reserved.

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Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany

 

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