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Horm Metab Res 2023; 55(06): 413-419
DOI: 10.1055/a-2044-9227
Original Article: Endocrine Research

CCND1-Induced Autophagy Contributes to Lymph Node Metastasis in Endometrial Cancer

Yi Su‡
1   Shandong University Cancer Center, Jinan, China
2   Department of Radiotherapy, Yantai Yuhuangding Hospital, Yantai, China
,
Haonan Zhou‡
3   The First Clinical Medical School, Gansu University of Chinese Medicine, Lanzhou, China
,
Zhao Ma‡
2   Department of Radiotherapy, Yantai Yuhuangding Hospital, Yantai, China
,
Jing Liu   *
4   Department of Pathology, Yantai Yuhuangding Hospital, Yantai, China
,
Chen Li   *
2   Department of Radiotherapy, Yantai Yuhuangding Hospital, Yantai, China
› Author Affiliations Funding Information The Science and Technology Project in Yantai — 2019YT0600074
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Correction: CCND1-Induced Autophagy Contributes to Lymph Node Metastasis in Endometrial CancerHorm Metab Res 2023; 55(06): e4-e4
DOI: 10.1055/a-2090-5663

Abstract

Endometrial cancer with lymph node metastasis shows poor prognosis, while the biomarker to predict the metastasis is lacking. The relative mRNA or protein expression of cyclin D1 (CCND1) and autophagy-related molecules were detected in real-time PCR and Western blot. Correlation analysis was applied to identify any significant patterns, and receiver operating characteristics (ROC) was performed to assess the prediction value. CCND1 vector was transfected in Ishikawa (ISK) cells, and the relative expression of autophagy-related molecules was detected with Western blot. CCND1 was overexpressed in endometrial cancer and correlated with lymph node metastasis. ROC analysis found that CCND1 had a predictive value to discriminate tumors from normal tissues (cut off=1.455; sensitivity, 71%; specificity, 84%; area under curve (AUC) 0.82; p<0.001) and had a predictive value to indicate metastasis (cut off=1.871; sensitivity, 54.17%; specificity, 75%; AUC 0.674; p=0.003). Increased BECLIN1 (r=0.39, p<0.001) and ATG5 (r=0.41, p<0.001) expression were positively correlated to CCND1. On the other hand, the relative protein expression of CCND1, BECLIN1, ATG5, ATG7, and LC3 I/II were also increased in tumor tissues. CCND1 overexpressed ISK cells showed upregulated BECLIN1, ATG5, ATG7, and LC3 I/II expression. CCND1 promoted autophagy may contribute to lymph node metastasis in endometrial cancer.

Key words

endometrial cancer - metastasis - autophagy

* Co-corresponding authors.




Publication History

Received: 01 December 2022

Accepted after revision: 23 February 2023

Article published online:
05 April 2023

© 2023. Thieme. All rights reserved.

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