Effects of High-Fructose Corn Syrup Intake on Glucocorticoid Metabolism in Rats During Childhood, Adolescence and Adulthood

Abstract

The consumption of high-fructose corn syrup (HFCS) has been increasing in recent decades, especially among children. Some reports suggest that children and adolescents are more sensitive to the adverse effects of fructose intake than adults. However, the underlying mechanism of the difference in vulnerability between adolescence and adulthood have not yet been elucidated. In this study, we attempted to elucidate the different effects of HFCS intake at different growth stages in rats: childhood and adolescence (postnatal day (PD) 21–60), young adulthood (PD60–100), and adulthood (PD100–140). Since alterations in hepatic glucocorticoid (GC) metabolism can cause diseases including insulin resistance, we focused on GC metabolizing enzymes such as 11 beta-hydroxysteroid dehydrogenase 1 and 2 (Hsd11b1 and Hsd11b2) and steroid 5 alpha-reductase 1 (Srd5a1). Western blotting showed an increase in Hsd11b1 expression and a decrease in Hsd11b2 expression in childhood and adolescence but not in adulthood. We also observed changes in Hsd11b1 and Hsd11b2 activities only in childhood and adolescence, consistent with the results of mRNA and protein expression analysis. The effect of high-fructose intake with regards to GC metabolism may therefore vary with developmental stage. This study provides insight into the adverse effects of fructose on GC metabolism in children in the context of increasing rates of HFCS consumption.

Key words

11 beta-hydroxysteroid dehydrogenase - steroid 5 alpha-reductase 1 - corticosterone - metabolic disorders - sugar

Introduction

Fructose is a monosaccharide found in fruits and vegetables and is often used in foods and beverages as high-fructose corn syrup (HFCS). The consumption of HFCS increased rapidly in the 1970s owing to its sweet taste and low price. Epidemiological and experimental evidence suggests that fructose ingestion is a risk factor for metabolic diseases, such as obesity, hypertension, dyslipidemia, and insulin resistance [1] [2] [3]. Recently, HFCS consumption has increased, especially in children [4]. Vos et al. showed that nearly one-fourth of the adolescent population obtain more than 15% of their calories from fructose [5]. Moreover, rates of adolescent obesity have been on the rise in recent years, from 1% in 1975 to 8% in 2016 [6]. Thus, there is a concern that HFCS intake during adolescence may contribute to increasing rates of certain metabolic disorders.

Adolescence is the period of organ development and physical growth from childhood to adulthood, defined as 10–19 years of age in humans and as postnatal days (PD) 21–60 in rodents [7]. The importance of the adolescent environment is becoming clearer; a growing body of evidence indicates that children and adolescents are more sensitive than adults to the effects of food intake/nutrition, such as fructose consumption. Hsu et al. showed more significant effects of fructose intake on brain function, metabolic outcomes, and neuroinflammation in adolescence than in adulthood [8]. In addition, impaired insulin signaling because of high-fructose intake has been observed during adolescence but not during adulthood [8] [9]. However, the causes of the difference in vulnerability between adolescence and adulthood has not yet been elucidated.

Glucocorticoids (GCs; corticosterone in rodents, cortisol in humans) are hormones important for the regulation of lipid and glucose metabolism and blood pressure [10] [11]. GCs are synthesized in the adrenal gland, and their synthesis is regulated by the hypothalamic-pituitary-adrenal axis. Synthesized GCs are regulated by 11 beta-hydroxysteroid dehydrogenase 1, 2 (Hsd11b1, Hsd11b2) and the steroid 5 alpha-reductase 1 (Srd5a1) enzymes [12] [13]. Hsd11b2 is mainly expressed in the kidney, while Srd5a1 is expressed in the liver [14] [15], and these enzymes convert active GC to inactive form [12] [13]. Hsd11b1 is mainly expressed in the liver and skeletal muscle, and regenerates active GC from its inactive form [16].

The alterations in the expression of GC metabolizing enzymes disrupt GC action and can cause disorders in the affected tissues [16]. For example, Hsd11b1 overexpression in the liver causes hepatic dysfunction [17], the deletion of Srd5a1 in mice causes insulin resistance and hepatic steatosis [14], and kidney-specific Hsd11b2 knock-out in mice leads to hypertension [18].

Fructose intake alters the expression of enzymes involved in GC metabolism [19] [20]. London et al. showed that high-fructose diets increased hepatic expression of Hsd11b1 [19]. Likewise, Vasiljević et al. reported that fructose-induced increase in Hsd11b1 expression may lead to disruption of insulin signaling [20]. These studies suggest the induction of abnormal GC metabolism due to fructose intake. Given that fructose intake before adulthood is harmful, fructose-induced metabolic abnormalities in GC may consequently be more pronounced in children and adolescents than in adulthood. However, previous studies have not considered the effects of fructose intake on individuals at different stages of maturation [19] [20]. To better understand the mechanism, fructose-induced disease pathogenesis needs to be analyzed based on the developmental stage.

In this study, we aimed to test the effects of 20% HFCS solution (containing 11% fructose) on GC metabolism at different developmental stages of experimental rats, including childhood and adolescence (PD21–60), young adulthood (PD60–100), and adulthood (PD100–140).

Materials and Methods

Animals

This study was approved by the Animal Ethics Committee of Fujita Health University (Permit No. H0862). Male Sprague-Dawley (SD) rats (SLC, Shizuoka, Japan) were obtained from Japan SLC (Hamamatsu, Japan) and kept under standard conditions at room temperature (23°C±3°C) under a 12:12 h light-dark cycle (the light period started at 8:00 am). In animal studies using rodents, adolescence is indicated as the period from weaning (PD21) to PD60. The period from PD60 to PD100 is defined as young adulthood [21]. In this study, the period after young adulthood was defined as adulthood. We divided the experimental period into three parts: childhood and adolescence (PD21–60; Period I), young adulthood (PD60–100; Period II), and adulthood (PD100–140; Period III) [22]. The control group (C, n=7–8) received distilled water for 40 days, whereas the HFCS-fed group (H, n=7–8) received 20% HFCS solution for the same period. A 20% aqueous HFCS solution was prepared using 75% HFCS (Japan Corn Starch, Tokyo, Japan). Standard chow (MF; Oriental Yeast, Tokyo, Japan) and water were available ad libitum to all animals throughout the experimental period. According to data from the Third United States National Health and Nutrition Examination Survey, the mean fructose consumption was>10% of total caloric intake and constituted approximately 20% of total caloric intake in the top 5% of fructose consumers [5]. As fructose is most commonly consumed in the form of HFCS, this study modeled conditions that relatively substituted the modern human diet. The body weight of the rats was measured every two weeks. At the end of the experimental period, the rats were fasted for 6 h before being perfused with saline and dissected under isoflurane anesthesia. The kidney, adrenal gland, gastrocnemius muscle, and liver were harvested and stored at −80°C until use.

Quantification of mRNA expression

Total RNA was isolated from the kidney, adrenal gland, gastrocnemius muscle, and liver using TRIzol Reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions [23]. Quantitative polymerase chain reaction (qPCR) was performed using the QuantStudio 7 Flex system (Thermo Fisher Scientific, Waltham, MA, USA) and the Thunderbird SYBR qPCR Mix (Toyobo, Osaka, Japan). Conditions were as follows: 95°C for 1 min and 40 cycles of 95°C for 15 s, 55°C for 30 s, and 72°C for 1 min. The target gene primers were designed by Fasmac Co., Ltd. (Kanagawa, Japan) and have been described previously [24]. The expression levels of target genes were normalized to the mRNA levels of actin beta (Actb) as an internal control. We calculated the fold changes in the expression between the control and HFCS-fed groups using the 2^-∆∆Ct method [25].

Western blotting

The kidney, adrenal gland, gastrocnemius muscle, and liver were homogenized in RIPA buffer (Wako Pure Chemicals, Osaka, Japan). In brief, the extracted protein were boiled in EzApply Buffer (Atto, Tokyo, Japan) for 3 min. Sodium dodecyl sulfate-treated proteins (20 μg) were separated by electrophoresis on a 12.5% polyacrylamide gel (Atto, Tokyo, Japan) and transferred onto membranes. (Atto, Tokyo, Japan) The membranes were incubated overnight at 4°C with primary antibodies against Hsd11b1 (ab39364; Abcam, Cambridge, UK), Hsd11b2 (14192–1-AP; Proteintech, Rosemont, USA), Srd5a1 (ab110123; Abcam, Cambridge, UK), and beta-actin (ab8227; Abcam, Cambridge, UK) [26]. Subsequently, the membranes were incubated with horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature. Immunoreactive bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore, Billerica, MA, USA). The intensities of specific chemiluminescence bands were analyzed using a Lumino image analyzer (ImageQuant LAS 3000; GE Healthcare, Amersham, UK).

Activities of 11 beta-hydroxysteroid dehydrogenase 1 and 2 (Hsd11b1and Hsd11b2) enzymes

Liver was homogenized to 40 mg/mL concentration in 30 mM Tris buffer (pH=7.4) containing 0.9 mM ethylenediaminetetraacetic acid and 0.3 mM sucrose. Reaction buffers A and B were then prepared. The activity of Hsd11b1 or Hsd11b2 was determined in buffer A or B, respectively. Reaction buffer A was 0.01 M sodium phosphate buffer containing 200 µM cortisone (Merck, Darmstadt, Germany) and 2 mM NADPH. Reaction buffer B contained 0.01 M sodium phosphate buffer containing 100 µM cortisol (VWR International Ltd., Leuven, Belgium) and 2 mM NAD⁺. Cortisone and cortisol were used as substrates for Hsd11b1 and Hsd11b2 because these steroids show high affinity with rat Hsd11b1 and Hsd11b2 [27] [28]. The reaction was initiated by adding the homogenate (equivalent to 10 mg of tissue). After incubation at 37°C for 30 min, the reaction buffer was extracted by adding 2 mL chloroform and 50 µM dexamethasone (Merck, Darmstadt, Germany) as an extrinsic control. The chloroform phase was transferred to a tube and evaporated at 45°C to dryness. The residue was dissolved in 60% methanol and fractionated using a high-performance liquid chromatography (HPLC) system (UV-2075 Intelligent UV/Vis Detector, Jasco Inc., Tokyo, Japan) to measure cortisone, cortisol, and dexamethasone [29]. The reverse-phase HPLC analysis was performed on a 5C₁₈-MS-II column (Nacalai Tesque, Kyoto, Japan) at a column temperature of 30°C and detection wavelength of 254 nm.

Statistical analysis

Statistical analyses were performed using JMP version 14 (SAS Institute, Cary, NC, USA). All data are expressed as the mean±standard deviation, and statistical analysis was performed using the Student’s t-test [30]. Statistical significance was defined as p<0.05.

Results

The experimental rats were fed distilled water or 20% HFCS solution for 40 days during childhood and adolescence (PD21–60), young adulthood (PD60–100), and adulthood (PD100–140) ([Fig. 1]). Assessment of the effects of HFCS intake on body weight during each developmental period showed no significant differences between these periods ([Fig. 1]).

Fig. 1 Experimental design. The experimental period was divided to assess rats in three stages of their growth: childhood and adolescence (postnatal day (PD) 21–60; Period I), young adulthood (PD60–100; Period II), and adulthood (PD100–140; Period III). Male rats were fed either distilled water or a 20% HFCS solution for 40 days.

To evaluate the effect of excess HFCS intake on GC metabolism in childhood and adolescence, we analyzed the mRNA expression of Hsd11b1 and Hsd11b2, and Srd5a1 in the kidney, adrenal gland, muscle, and liver. Renal Hsd11b2 levels were lowered during Period I ([Table 1]). In the liver, Hsd11b1 levels increased, and levels of Hsd11b2 and Srd5a1 decreased during Period I. Hepatic Hsd11b1 and Hsd11b2 expression was not significantly different in Periods II and III, and Srd5a1 expression decreased during all periods ([Fig. 2]).

Fig. 2 Effect of HFCS intake on hepatic mRNA levels during each period. Hsd11b1, Hsd11b2, and Srd5a1 mRNA levels were quantified by quantitative polymerase chain reaction. The results are expressed as the ratio of the relative intensity of the gene expression levels to that of Actin beta. C, control group (n=7–8); H, HFCS-fed group (n=7–8). Values are presented as mean±standard deviation. *p<0.05.

Table 1 List of qPCR analysis of corticosterone metabolizing enzyme.

	Period I		Period II		Period III
	C		C		C
mRNA	Relative mRNA Level		Relative mRNA Level		Relative mRNA Level
Liver
Hsd11b1	1.00±0.16	1.57±0.38*	1.00±0.36	1.09±0.22	1.00±0.45	0.78±0.27
Hsd11b2	1.00±0.31	0.62±0.27*	1.00±0.23	0.83±0.39	1.00±0.26	0.95±0.11
Srd5a1	1.00±0.29	0.51±0.12*	1.00±0.27	0.69±0.34*	1.00±0.43	0.56±0.22*
Kidney
Hsd11b1	1.00±0.29	0.73±0.26	1.00±0.12	1.02±0.28	1.00±0.15	1.13±0.27
Hsd11b2	1.00±0.23	0.52±0.09*	1.00±0.22	0.98±0.20	1.00±0.22	0.88±0.28
Srd5a1	1.00±0.23	0.77±0.40	1.00±0.13	0.92±0.21	1.00±0.16	0.91±0.26
Adrenal Gland
Hsd11b1	1.00±0.23	1.75±0.86	1.00±0.23	1.00±0.25	1.00±0.42	0.99±0.23
Hsd11b2	1.00±0.31	0.94±0.11	1.00±0.24	1.02±0.23	1.00±0.26	1.24±0.19
Srd5a1	1.00±0.20	1.43±0.61	1.00±0.17	0.97±0.22	1.00±0.40	1.16±0.26
Muscle
Hsd11b1	1.00±0.46	0.81±0.21	1.00±0.30	1.17±0.43	1.00±0.22	1.10±0.21
Hsd11b2	1.00±0.50	1.06±0.42	1.00±0.64	0.61±0.38	1.00±0.79	1.15±0.25
Srd5a1	1.00±0.31	0.73±0.40	1.00±0.50	1.40±0.57	1.00±0.41	0.74±0.18

Values are presented as mean ± standard deviation. Abbreviations: 11 beta-hydroxysteroid dehydrogenase 1, 2 (Hsd11b1, Hsd11b2); steroid 5 alpha-reductase 1 (Srd5a1). C: control group; H: HFCS-fed group. HFCS: high-fructose corn syrup *p<0.05.

We also quantified Hsd11b1, Hsd11b2, and Srd5a1 protein levels in these tissues. The kidney, adrenal gland, and muscle did not show any significant changes ([Table 2]). Following hepatic mRNA expression, Hsd11b1 protein levels increased significantly, and Hsd11b2 protein levels decreased significantly during Period I (p=0.05), but the levels did not differ significantly during Periods II and III ([Fig. 3]). In addition, hepatic Srd5a1 protein levels decreased during Period I, but no difference was observed during Periods II and III ([Fig. 3]).

Fig. 3 Effect of HFCS intake on hepatic protein levels during each period. Hsd11b1, Hsd11b2, and Srd5a1 protein levels were quantified using western blotting. Representative samples of western blot images are shown. The results were expressed as a ratio of the relative intensity of protein expression to that of Actin beta. C: control group (n=4–6); H: HFCS-fed group (n=4–6). Values are presented as mean±standard deviation. *p<0.05.

Table 2List of western blotting analysis of corticosterone metabolizing enzymes.

	Period I		Period II		Period III
	C		C		C
Protein	Relative Protein Level		Relative Protein Level		Relative Protein Level
Liver
Hsd11b1	1.00±0.09	1.20±0.12*	1.00±0.14	1.04±0.09	1.00±0.26	1.04±0.39
Hsd11b2	1.00±0.06	0.82±0.10*	1.00±0.20	0.88±0.13	1.00±0.34	0.93±0.25
Srd5a1	1.00±0.10	0.73±0.13*	1.00±0.18	0.79±0.13	1.00±0.09	0.93±0.16
Kidney
Hsd11b1	1.00±0.27	0.97±0.35	1.00±0.07	1.01±0.14	1.00±0.17	0.98±0.07
Hsd11b2	1.00±0.25	1.01±0.27	1.00±0.04	1.01±0.11	1.00±0.31	0.97±0.26
Srd5a1	1.00±0.23	1.11±0.31	1.00±0.21	1.09±0.20	1.00±0.23	0.85±0.15
Adrenal Gland
Hsd11b1	1.00±0.08	0.99±0.41	1.00±0.35	0.96±0.20	1.00±0.30	1.02±0.40
Hsd11b2	1.00±0.19	0.95±0.19	1.00±0.27	1.00±0.22	1.00±0.18	1.04±0.19
Srd5a1	1.00±0.21	1.09±0.20	1.00±0.03	0.96±0.10	1.00±0.22	0.94±0.12
Muscle
Hsd11b1	1.00±0.21	0.99±0.15	1.00±0.20	0.83±0.28	1.00±0.25	1.17±0.29
Hsd11b2	1.00±0.34	1.01±0.14	1.00±0.32	1.04±0.22	1.00±0.39	1.25±0.39
Srd5a1	1.00±0.41	1.14±0.29	1.00±0.28	0.82±0.41	1.00±0.33	0.90±0.26

Values are presented as mean ± standard deviation. Abbreviations: 11 beta-hydroxysteroid dehydrogenase 1, 2 (Hsd11b1, Hsd11b2); steroid 5 alpha-reductase 1 (Srd5a1). C: Control group; H: HFCS-fed group. HFCS: high-fructose corn syrup *p<0.05.

We analyzed the activities of Hsd11b1 and Hsd11b2 enzymes that are likely to be highly sensitive to GC metabolism in each period ([Fig. 4]). As expected, Hsd11b1 enzyme activity increased while that of Hsd11b2 enzyme decreased during Period I, but no difference was observed during Periods II and III. The enzymatic activity of hepatic Hsd11b1 and Hsd11b2 were 10.4±2.5 and 11.0±0.5 pmol/min/mg tissue in the control group, respectively.

Fig. 4 Effect of HFCS intake on hepatic Hsd11b1 and Hsd11b2 enzyme activities during each period. Hepatic Hsd11b1 and Hsd11b2 enzyme activities according to HFCS intake are shown. Hsd11b1 activity was calculated based on the rate of conversion of cortisone to cortisol. Hsd11b2 activity was calculated based on the rate of conversion of cortisol to cortisone. These metabolites were measured using high-performance liquid chromatography. C: control group (n=5–7); H: HFCS-fed group (n=4–7). Values are presented as mean±standard deviation. *p<0.05.

Discussion

We analyzed the effects of HFCS intake on GC metabolism in the kidney, adrenal gland, muscle, and liver at different developmental stages. HFCS-induced increase in hepatic Hsd11b1 activity was observed only in the liver in Period I. Similarly, the reduction of hepatic Hsd11b2 activity was also induced only in Period I. Thus, our study shows that the effects of HFCS in relation to GC metabolism may vary with the developmental period.

The significant effects of GC exposure are reported more in adolescence than in adulthood. For example, Kinlein et al. observed chronic GC exposure-induced reduction in bone density in adolescence but not in adulthood [31]. According to our enzyme activity analysis, GC did not seem to accumulate in the liver during Periods II and III, although its accumulation may increase during Period I. Adverse effects of high-fructose intake were only observed in adolescence [8] [9]. This phenomenon may be partially explained by the presence or absence of abnormal hepatic GC metabolism.

In this study, we observed increased Hsd11b1 activity in the rat liver in Period I but not in Periods II and III. Liver-specific Hsd11b1 overexpressing mice display insulin resistance and dyslipidemia [17], while Hsd11b1 knock-out mice are protected from metabolic disorders [32]. These reports may support our finding that accumulated active GC in the liver exacerbates the adverse effects of fructose in adolescence. We observed reduced hepatic Hsd11b2 activity by approximately 50% after HFCS intake during Period I. Hsd11b2 is mainly expressed in the kidney; however, Chia et al. showed that Hsd11b2 enzyme activity in the liver is about 70% of that in the kidney [33]. The decrease in Hsd11b2 activity in the liver may contribute to the increase in active GC. Moreover, we observed a reduction in hepatic Srd5a1 activity in Period I. In one study, the affinity of the Srd5a1 enzyme for GC was 1000-fold lower than that of Hsd11b2 [34] [35], implying that the decrease in Srd5a1 activity may not contribute to GC inactivation compared to Hsd11b2 or has only a negligible effect.

Our results implied that adolescents are more vulnerable to environmental changes than adults and are more likely to experience negative consequences. However, several animal experiments have shown that adverse effects of fructose intake occur not only in adolescence but also in adulthood. This discrepancy in findings may be explained by differences in the concentration of fructose used. Some studies regarding the adverse effects of fructose have been conducted using 60–70% fructose concentrations [36] [37], which is an unlikely concentration to be ingested as part of a modern diet. Another possible explanation is the difference in the duration of the intake period. We administered the HFCS solution for 40 days, unlike some studies in which adult rats were fed 10% fructose for a longer time [38] [39]. For example, Ibrahim et al. reported that adult rats fed a 10% fructose solution in drinking water for 16 weeks showed increased serum glucose and insulin levels [38]. Notably, these reports have observed body weight gain, which differs from the results of our study (Supplemental Fig. 1). In summary, the lack of significant effects of HFCS during adulthood in this study may be because of differences in the concentration of fructose administration and length of the intake period.

According to our previous studies, exposure to fructose during early life stages such as fetal and lactation induces changes in miRNA expression and DNA methylation level in the liver [23] [40]. These epigenetic modifications may be maintained at least until adulthood, altering mRNA expression of genes related to lipid metabolism. These reports suggest that the effects on GC-related gene expression changes may be observed via epigenetic changes even after HFCS intake is discontinued. Further study is needed to analyze this point.

The current study has a few limitations. First, we did not clarify the mechanism of the transcriptional changes in GC metabolizing enzymes in adolescent HFCS intake. Cooper et al. reported that interleukin-1 beta (Il1b) and tumor necrosis factor-alpha (TNFa) decrease Hsd11b2 and increase Hsd11b1 expression and activity [41]. Therefore, we analyzed Il1b and TNFa mRNA levels but did not observe any increase in their levels (data not shown). Further analysis is needed to determine the mechanism of the change in GC metabolizing enzymes in adolescent HFCS intake. Next, this study only analyzed GC metabolism in the liver and not steroid levels. Fructose intake increases GC levels, but given that increased GC in the liver is associated with abnormal lipid metabolism and insulin resistance [42], it is necessary to measure steroid levels.

In conclusion, we showed that HFCS intake during adolescence may induce hepatic GC accumulation. This study provides insight into the adverse effects of fructose on GC metabolism in children in the context of increasing rates of HFCS consumption.

Conflict of Interest

The authors declare that they have no conflict of interest.

• References

• 1 Yamazaki M, Munetsuna E, Yamada H. et al. Fructose consumption induces hypomethylation of hepatic mitochondrial DNA in rats. Life Sci 2016; 149: 146-152
• 2 Ohashi K, Munetsuna E, Yamada H. et al. High fructose consumption induces DNA methylation at PPARalpha and CPT1A promoter regions in the rat liver. Biochem Biophys Res Commun 2015; 468: 185-189
• 3 Dornas WC, de Lima WG, Pedrosa ML. et al. Health implications of high-fructose intake and current research. Adv Nutr 2015; 6: 729-737
• 4 Hemmingsson E. Early childhood obesity risk factors: Socioeconomic adversity, family dysfunction, offspring distress, and junk food self-medication. Curr Obes Rep 2018; 7: 204-209
• 5 Vos MB, Kimmons JE, Gillespie C. et al. Dietary fructose consumption among US children and adults: The Third National Health and Nutrition Examination Survey. Medscape J Med 2008; 10: 160
• 6 Collaboration NCDRF. Worldwide trends in body-mass index, underweight, overweight, and obesity from 1975 to 2016: a pooled analysis of 2416 population-based measurement studies in 128.9 million children, adolescents, and adults. Lancet 2017; 390: 2627-2642
• 7 Azogu I, Cossette I, Mukunzi J. et al. Sex-specific differences in adult cognition and neuroplasticity following repeated combinatory stress and TrkB receptor antagonism in adolescence. Horm Behav 2019; 113: 21-37
• 8 Hsu TM, Konanur VR, Taing L. et al. Effects of sucrose and high fructose corn syrup consumption on spatial memory function and hippocampal neuroinflammation in adolescent rats. Hippocampus 2015; 25: 227-239
• 9 Crescenzo R, Cigliano L, Mazzoli A. et al. Early effects of a low fat, fructose-rich diet on liver metabolism, insulin signaling, and oxidative stress in young and adult rats. Front Physiol 2018; 9: 411
• 10 Macfarlane DP, Forbes S, Walker BR. Glucocorticoids and fatty acid metabolism in humans: Fuelling fat redistribution in the metabolic syndrome. J Endocrinol 2008; 197: 189-204
• 11 Moraitis AG, Block T, Nguyen D. et al. The role of glucocorticoid receptors in metabolic syndrome and psychiatric illness. J Steroid Biochem Mol Biol 2017; 165: 114-120
• 12 Morgan SA, Hassan-Smith ZK, Doig CL. et al. Glucocorticoids and 11beta-HSD1 are major regulators of intramyocellular protein metabolism. J Endocrinol 2016; 229: 277-286
• 13 Tomlinson JW, Walker EA, Bujalska IJ. et al. 11beta-hydroxysteroid dehydrogenase type 1: A tissue-specific regulator of glucocorticoid response. Endocr Rev 2004; 25: 831-866
• 14 Livingstone DE, Barat P, Di Rollo EM. et al. 5alpha-Reductase type 1 deficiency or inhibition predisposes to insulin resistance, hepatic steatosis, and liver fibrosis in rodents. Diabetes 2015; 64: 447-458
• 15 Romero DG, Zhou M, Gomez-Sanchez CE. Cloning and expression of the bovine 11beta-hydroxysteroid dehydrogenase type-2. J Steroid Biochem Mol Biol 2000; 72: 231-237
• 16 Odermatt A, Kratschmar DV. Tissue-specific modulation of mineralocorticoid receptor function by 11beta-hydroxysteroid dehydrogenases: An overview. Mol Cell Endocrinol 2012; 350: 168-186
• 17 Paterson JM, Morton NM, Fievet C. et al. Metabolic syndrome without obesity: Hepatic overexpression of 11beta-hydroxysteroid dehydrogenase type 1 in transgenic mice. Proc Natl Acad Sci U S A 2004; 101: 7088-7093
• 18 Ueda K, Nishimoto M, Hirohama D. et al. Renal dysfunction induced by kidney-specific gene deletion of Hsd11b2 as a primary cause of salt-dependent. hypertension. Hypertension 2017; 70: 111-118
• 19 London E, Castonguay TW. High fructose diets increase 11beta-hydroxysteroid dehydrogenase type 1 in liver and visceral adipose in rats within 24-h exposure. Obesity (Silver Spring) 2011; 19: 925-932
• 20 Vasiljevic A, Bursac B, Djordjevic A. et al. Hepatic inflammation induced by high-fructose diet is associated with altered 11betaHSD1 expression in the liver of Wistar rats. Eur J Nutr 2014; 53: 1393-1402
• 21 Hodges TE, Lee GY, Noh SH. et al. Sex and age differences in cognitive bias and neural activation in response to cognitive bias testing. Neurobiol Stress 2022; 18: 100458
• 22 Kageyama I, Yamada H, Munetsuna E. et al. Differential effects of excess high-fructose corn syrup on the DNA methylation of hippocampal neurotrophic factor in childhood and adolescence. PLoS One 2022; 17: e0270144
• 23 Ando Y, Yamada H, Munetsuna E. et al. Maternal high-fructose corn syrup consumption causes insulin resistance and hyperlipidemia in offspring via DNA methylation of the Pparα promoter region. J Nutr Biochem 2022; 103: 108951
• 24 Munetsuna E, Yamada H, Yamazaki M. et al. Maternal high-fructose intake increases circulating corticosterone levels via decreased adrenal corticosterone clearance in adult offspring. J Nutr Biochem 2019; 67: 44-50
• 25 Mizuno G, Munetsuna E, Yamada H. et al. Maternal fructose consumption downregulates hippocampal catalase expression via DNA methylation in rat offspring. Nutr Res 2021; 92: 40-48
• 26 Yamada H, Munetsuna E, Yamazaki M. et al. Maternal fructose-induced oxidative stress occurs via Tfam and Ucp5 epigenetic regulation in offspring hippocampi. FASEB J 2019; 33: 11431-11442
• 27 Prince PD, Santander YA, Gerez EM. et al. Fructose increases corticosterone production in association with NADPH metabolism alterations in rat epididymal white adipose tissue. J Nutr Biochem 2017; 46: 109-116
• 28 Balazs Z, Schweizer RA, Frey FJ. et al. DHEA induces 11 -HSD2 by acting on CCAAT/enhancer-binding proteins. J Am Soc Nephrol 2008; 19: 92-101
• 29 Jamieson PM, Walker BR, Chapman KE. et al. 11 beta-hydroxysteroid dehydrogenase type 1 is a predominant 11 beta-reductase in the intact perfused rat liver. J Endocrinol 2000; 165: 685-692
• 30 Yamazaki M, Yamada H, Munetsuna E. et al. Excess maternal fructose consumption impairs hippocampal function in offspring via epigenetic modification of BDNF promoter. FASEB J 2018; 32: 2549-2562
• 31 Kinlein SA, Shahanoor Z, Romeo RD. et al. Chronic corticosterone treatment during adolescence has significant effects on metabolism and skeletal development in male C57BL6/N mice. Endocrinology 2017; 158: 2239-2254
• 32 Kotelevtsev Y, Holmes MC, Burchell A. et al. 11beta-hydroxysteroid dehydrogenase type 1 knockout mice show attenuated glucocorticoid-inducible responses and resist hyperglycemia on obesity or stress. Proc Natl Acad Sci U S A 1997; 94: 14924-14929
• 33 Chia YY, Ton SH, Kadir KB. Effects of glycyrrhizic acid on 11 beta-hydroxysteroid dehydrogenase (11 betaHSD1 and 2) activities and HOMA-IR in rats at different treatment periods. Exp Clin Endocrinol Diabetes 2010; 118: 617-624
• 34 Kuhlmann K, Buhler H, Ragosch V. et al. Kinetic studies on rabbit liver glucocorticoid 5alpha-reductase. Horm Metab Res 2000; 32: 20-25
• 35 Naray-Fejes-Toth A, Fejes-Toth G. Subcellular localization of the type 2 11beta-hydroxysteroid dehydrogenase. A green fluorescent protein study. J Biol Chem 1996; 271: 15436-15442
• 36 Huang D, Dhawan T, Young S. et al. Fructose impairs glucose-induced hepatic triglyceride synthesis. Lipids Health Dis 2011; 10: 20
• 37 Kawasaki T, Igarashi K, Koeda T. et al. Rats fed fructose-enriched diets have characteristics of nonalcoholic hepatic steatosis. J Nutr 2009; 139: 2067-2071
• 38 Ibrahim SM, El-Denshary ES, Abdallah DM. Geraniol, alone and in combination with pioglitazone, ameliorates fructose-induced metabolic syndrome in rats via the modulation of both inflammatory and oxidative stress status. PLoS One 2015; 10: e0117516
• 39 Mahmoud AA, Elshazly SM. Ursodeoxycholic acid ameliorates fructose-induced metabolic syndrome in rats. PLoS One 2014; 9: e106993
• 40 Munetsuna E, Yamada H, Yamazaki M. et al. Maternal fructose intake predisposes rat offspring to metabolic disorders via abnormal hepatic programming. FASEB J 2021; 35: e22030
• 41 Cooper MS, Bujalska I, Rabbitt E. et al. Modulation of 11beta-hydroxysteroid dehydrogenase isozymes by proinflammatory cytokines in osteoblasts: an autocrine switch from glucocorticoid inactivation to activation. J Bone Miner Res 2001; 16: 1037-1044
• 42 Elakovic I, Kovacevic S, Vojnovic Milutinovic D. et al. Fructose consumption affects glucocorticoid signaling in the liver of young female rats. Nutrients 2020; 12

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Artikel online veröffentlicht:
11. November 2022