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Exp Clin Endocrinol Diabetes 2020; 128(03): 164-169
DOI: 10.1055/a-0603-3539
Article
© Georg Thieme Verlag KG Stuttgart · New York

Assessment of the Dynamic Insulin Secretion and Sensitivity Test (DISST) Pre and Post Gastric bypass Surgery

John Wilson
1   Department of Medicine, University of Otago Wellington, Wellington, New Zealand
,
Paul Docherty
2   Centre for Bioengineering, University of Canterbury, Christchurch, New Zealand
,
Richard Stubbs
3   P3 Research Limited, Wellington, New Zealand
,
J Geoffrey Chase
2   Centre for Bioengineering, University of Canterbury, Christchurch, New Zealand
,
Jeremy Krebs
1   Department of Medicine, University of Otago Wellington, Wellington, New Zealand
› Author Affiliations Funding JW received salary funding from a New Zealand Diabetes Foundation Fellowship. Grant in aid from Wellington Medical Research Foundation for funding of Glucose analyser and sample analysis.
Further Information

Publication History

received 22 November 2017
revised 25 March 2018

accepted 09 April 2018

Publication Date:
11 June 2018 (online)

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Abstract

Objective To compare the dynamic insulin secretion and sensitivity test (DISST) with the euglycaemic clamp in individuals undergoing open Roux-en-Y gastric bypass (RYGB) surgery prior-to and one month after surgery.

Methods Insulin sensitivity in individuals with obesity undergoing RYGB was studied with DISST and a euglycaemic hyperinsulinaemic clamp.

Results Eleven participants, including nine females, mean(SD) age 51.2 (12.1) yrs, with a preoperative BMI of 48.7(9.5)kg/m2 were studied. Weight reduced from a mean (SD) of 133.8 (29.8) kg to 123.8 (28.9) kg post-surgery (p<0.001). The mean (SD) insulin sensitivity index (ISI-DISST) was 3.07×10−4 (2.18) L.pmol−1.min−1 preoperatively and 2.36 ×10−4 (0.78)L.pmol−1.min−1 postoperatively (p=0.37). The mean(SD) clamp ISI was 2.14 ×10−2 (1.80) mg.L.kg−1.min−1.pmol−1 and 2.00×10−2.(0.76) mg.L.kg−1.min−1.pmol−1 postoperatively (p=0.86). Correlation between ISI-DISST and ISI-Clamp preoperatively was r=0.81(95%CI 0.37-0.95) and post-operatively r=0.47(95%CI 0-0.88). Bland-Altman analysis demonstrates systematic bias between the two tests, where DISST underestimated insulin sensitivity compared with the clamp by 0.96×10-2.mg.L.kg−1.min−1.pmol−1 (95%CI -2.24 to 0.32).

Conclusions There was a strong correlation between DISST and the clamp preoperatively and DISST can be used to estimate insulin sensitivity in individuals with morbid obesity. After RYGB surgery, DISST had a weaker correlation with the clamp suggesting the fundamental physiological determinants of insulin sensitivity being measured by each method change in different ways with changes in glucose homeostasis following RYGB surgery.

Key words

dynamic insulin secretion and sensitivity test - Roux-en-Y gastric bypass - obesity - weight loss - Type 2 Diabetes
 

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