Subscribe to RSS
DOI: 10.1016/j.homp.2008.11.011
Lymphocyte proliferation stimulated by activated human macrophages treated with Canova
Subject Editor:
Publication History
Received26 February 2008
revised17 October 2008
accepted18 November 2008
Publication Date:
20 December 2017 (online)
Introduction: Canova (CA) is a homeopathic medication with immunomodulatory properties, recommended for patients with a depressed immune system. CA has been reported to increase in leukocyte numbers, cellular differentiation and reduction in tumor size.
Aim and method: Since CA may stimulate lymphocyte differentiation, proliferation, and/or survival, the aim of the present study was to compare the mitotic index (MI) of phytohemagglutinin-stimulated human lymphocytes cultured in a medium supplemented with human macrophages activated by CA, with lymphocytes cultured in a medium without CA-treated macrophages.
Results: In this study, the MI of lymphocyte cultured received the medium containing CA-stimulated macrophages showed a higher proliferation index (p < 0.01) than the lymphocytes cultured in a medium without CA-treated macrophages. Our results suggest that CA treatment, in addition to activating macrophages, indirectly induces lymphocyte proliferation and has potential as a new adjuvant therapeutic approach.
-
References
- 1 Cesar B., Abud A.P., de Oliveira C.C. et al. Activation of mononuclear bone marrow cells treated in vitro with a complex homeopathic medication. Mícron 2008; 39: 461-470.
- 2 Piemonte M.R., Buchi D.F. Analysis of IL-2, IFN-gamma and TNF-alpha production, alpha5 beta1 integrins and actin filaments distribution in peritoneal mouse macrophages treated with homeopathic medicament. J Submicrosc Cytol Pathol 2002; 34: 255-263.
- 3 De Oliveira C.C., de Oliveira S.M., Godoy L.M. et al. Canova, a Brazilian medical formulation, alters oxidative metabolism of mice macrophages. J Infect 2006; 52: 420-432.
- 4 Seligmann I.C., Lima P.D., Cardoso P.C. et al. The anticancer homeopathic composite “Canova Method” is not genotoxic for human lymphocytes in vitro. Genet Mol Res 2003; 2: 223-228.
- 5 Janossy G., Greaves M.F. Lymphocyte activation II. Discriminating stimulation of lymphocyte subpopulations by phytomitogens and heterologous antilymphocyte sera. Clin Exp Immunol 1972; 10: 525-536.
- 6 Preston R.J., San Sebastian J.R., McFee A.F. The in vitro human lymphocyte assay for assessing the clastogenicity of chemical agents. Mutat Res 1987; 189: 175-183.
- 7 Amorim M.I.M., Mergler D., Bahia M.O. et al. Cytogenetic damage related to low levels of methyl mercury contamination in the Brazilian Amazon. An Acad Bras Cienc 2000; 72: 487-507.
- 8 Moorhead P.S., Nowell P.C., Mellman W.J. et al. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res 1960; 20: 613-616.
- 9 Makrides C., Koukouvas M., Achillews G. et al. Methomyl-induced severe acute pancreatitis: possible etiological association. J Pancreas 2005; 6: 166-171.
- 10 Sato D.Y., Wal R., de Oliveira C.C. et al. Histopathological and immunophenotyping studies on normal and sarcoma 180-bearing mice treated with a complex homeopathic medication. Homeopathy 2005; 94: 26-32.
- 11 Abud A.P., Cesar B., Cavazzani L.F. et al. Activation of bone marrow cells treated with Canova in vitro. Cell Biol Int 2006; 30: 808-816.
- 12 Roitt I., Brostoff J., Male D. Immunology. New York: Mosby; 2001.
- 13 Lima P.D., Leite D.S., Vasconcellos M.C. et al. Genotoxic effects of aluminum chloride in cultured human lymphocytes treated in different phases of cell cycle. Food Chem Toxicol 2007; 45: 1154-1159.