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CC BY-NC-ND 4.0 · Eur J Dent 2016; 10(01): 092-096
DOI: 10.4103/1305-7456.175691
Original Article
Dental Investigation Society

Investigation of eluted monomers from resin-based root canal sealer by high-performance liquid chromatography analysis

Huma Omurlu
1   Department of Restorative and Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkiye
,
Hacer Deniz Arisu
1   Department of Restorative and Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkiye
,
Evrim Eliguzeloglu Dalkilic
2   Department of Restorative Dentistry, Faculty of Dentistry, Yeni Yüzyıl University, İstanbul, Turkiye
,
Ugur Tamer
3   Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara, Turkiye
,
Hilal Torul
3   Department of Analytical Chemistry, Faculty of Pharmacy, Gazi University, Ankara, Turkiye
› Author Affiliations
Further Information

Publication History

Publication Date:
23 September 2019 (online)

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ABSTRACT

Objective: The purpose of the current study was to determine the amount of urethane dimethacrylate (UDMA), bisphenol A-glycidyl methacrylate (Bis-GMA), poly (ethylene glycol) dimethacrylate (PEGDMA), bisphenol A ethoxylated dimethacrylate (Bis-EMA), and 2-hydroxyethyl methacrylate (HEMA) eluted from resin-based root canal sealer, epiphany, using high-performance liquid chromatography (HPLC). Materials and Methods: Epiphany was placed into the plastic molds and light-cured with a light emitting diode. After the curing process, each specimen in the first group (n = 12) was immersed in Eppendorf tubes containing a phosphate-buffered saline solution (PBS) and incubated for 45 s. In the second group, each specimen (n = 12) was immersed in Eppendorf tubes containing PBS and incubated for 24 h. Of the specimen extracts, 100 μL were subjected to HPLC. Analysis of data was accomplished with one-way analysis of variance (P < 0.05). Results: All of the samples eluted HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA. A significant difference was determined between the time periods of HEMA, UDMA, PEGDMA, and Bis-EMA (P < 0.05). Conclusion: The results of the current study showed that Epiphany releases HEMA, UDMA, Bis-GMA, PEGDMA, and Bis-EMA in both time periods.

Key words:

High-performance liquid chromatography - monomer - resin - sealer
 

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