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CC BY-NC-ND 4.0 · Eur J Dent 2015; 09(01): 006-010
DOI: 10.4103/1305-7456.149630
Original Article
Dental Investigation Society

Comparison of cytotoxicity of various concentrations origanum extract solution with 2% chlorhexidine gluconate and 5.25% sodium hypochlorite

Evren Ok
1   Department of Endodontics, Faculty of Dentistry, Sifa University, Izmir, Turkiye
,
Necdet Adanir
2   Department of Endodontics, Faculty of Dentistry, Suleyman Demirel University, Isparta, Turkiye
,
Sema Hakki
3   Department of Periodontology, Faculty of Dentistry, Selcuk University, Konya, Turkiye
› Author Affiliations
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Publication History

Publication Date:
04 September 2019 (online)

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ABSTRACT

Objective: The purpose of this in vitro study was to compare the cytotoxicity of 0.5–4.5 origanum extract solution (OES), 2% chlorhexidine gluconate (CHX) and 5.25% sodium hypochlorite (NaOCl) with WST-1 test on human periodontal ligament (hPDL) fibroblasts. Materials and Methods: About 0.5–4.5% OES, 2% CHX and 5.25% NaOCl solutions cytotoxicity was evaluated with cell culture test using PDL fibroblasts. Viability of hPDL cells was evaluated with WST-1 (Cell Proliferation Reagent WST-1 Roche) test at 1, 24 and 72nd h. hPDL cells were plated at 20 × 103 cells per well in 96-well plates. Absorbance values were read in optical density 480 nm by ELISA plate reader spectrophotometer. The statistical differences between various groups were evaluated using one-way ANOVA, post-hoc Duncan's Multiple Range test using SAS software. Statistically, a significant difference was considered at P < 0.001. Results: According to the 1-h cytotoxicity results, 0.5% OES showed the least cytotoxic effect in test groups. There were not found any statistical significance between 1% OES and 2% CHX. About 5.25% NaOCl showed more cytotoxic effect than 1% OES and 2% CHX. In 24 and 72 h, different concentrations of OES, 5.25% NaOCl, 2% CHX solutions showed similar cytotoxic effect. Conclusions: Based on these results, 1% OES and 2% CHX showed similar results and less cytotoxic effect than 5.25% NaOCl. It could be considered as a favorable solution concentration when OES was used as root canal irrigation solution.

Key words:

Chlorhexidine gluconate - cytotoxicity tests - origanum extract solution - sodium hypochlorite - WST-1 test
 

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