Plant Biol (Stuttg) 2006; 8(6): 813-820
DOI: 10.1055/s-2006-924177
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Fluorescence Labelling of Phosphatase Activity in Digestive Glands of Carnivorous Plants

B. J. Płachno1 , L. Adamec2 , I. K. Lichtscheidl3 , M. Peroutka3 , W. Adlassnig3 , J. Vrba4
  • 1Department of Plant Cytology and Embryology, The Jagiellonian University, Grodzka 52, 31-044 Cracow, Poland
  • 2Institute of Botany, Academy of Sciences of the Czech Republic, Section of Plant Ecology, Dukelská 135, 379 82 Třeboň, Czech Republic
  • 3Cell Imaging and Ultrastructure Research Unit, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
  • 4Hydrobiological Institute, Academy of Sciences of the Czech Republic, Na Sádkách 7, 370 05 České Budějovice, Czech Republic
Further Information

Publication History

Received: September 29, 2005

Accepted: April 5, 2006

Publication Date:
24 July 2006 (online)

Abstract

A new ELF (enzyme labelled fluorescence) assay was applied to detect phosphatase activity in glandular structures of 47 carnivorous plant species, especially Lentibulariaceae, in order to understand their digestive activities. We address the following questions: (1) Are phosphatases produced by the plants and/or by inhabitants of the traps? (2) Which type of hairs/glands is involved in the production of phosphatases? (3) Is this phosphatase production a common feature among carnivorous plants or is it restricted to evolutionarily advanced species? Our results showed activity of the phosphatases in glandular structures of the majority of the plants tested, both from the greenhouse and from sterile culture. In addition, extracellular phosphatases can also be produced by trap inhabitants. In Utricularia, activity of phosphatase was detected in internal glands of 27 species from both primitive and advanced sections and different ecological groups. Further positive reactions were found in Genlisea, Pinguicula, Aldrovanda, Dionaea, Drosera, Drosophyllum, Nepenthes, and Cephalotus. In Utricularia and Genlisea, enzymatic secretion was independent of stimulation by prey. Byblis and Roridula are usually considered as “proto-carnivores”, lacking digestive enzymes. However, we found high activity of phosphatases in both species. Thus, they should be classified as true carnivores. We suggest that the inflorescence of Byblis and some Pinguicula species might also be an additional “carnivorous organ”, which can trap a prey, digest it, and finally absorb available nutrients.

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B. J. Płachno

Department of Plant Cytology and Embryology
The Jagiellonian University

Grodzka 52

31-044 Cracow

Poland

Email: bartek78pl@poczta.onet.pl

Guest Editor: S. Porembski