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Plant Biol (Stuttg) 2006; 8(4): 407-418
DOI: 10.1055/s-2006-923961
Review Article

Georg Thieme Verlag Stuttgart KG · New York

Concepts and Approaches Towards Understanding the Cellular Redox Proteome

E. Ströher1 , K.-J. Dietz1
  • 1Faculty of Biology - W5-134, Bielefeld University, Universitätsstraße 25, 33501 Bielefeld, Germany
Weitere Informationen

Publikationsverlauf

Received: September 23, 2005

Accepted: January 25, 2006

Publikationsdatum:
11. Mai 2006 (online)

   Lizenzen und Reprints

Abstract

The physiological activity of a significant subset of cell proteins is modified by the redox state of regulatory thiols. The cellular redox homeostasis depends on the balance between oxidation of thiols through oxygen and reactive oxygen species and reduction by thiol-disulfide transfer reactions. Novel and improved methodology has been designed during recent years to address the level of thiol/disulfide regulation on a genome-wide scale. The approaches are either based on gel electrophoresis or on chromatographic techniques coupled to high end mass spectrometry. The review addresses diagonal 2D‐SDS-PAGE, targeted identification of specific redox-interactions, affinity chromatography with thioredoxins and glutaredoxins, gel-based and non-gel based labelling techniques with fluorophores (such as Cy3, Cy5, ICy), radioisotopes, or with isotope-coded affinity tags (ICAT), differential gel electrophoresis (DIGE) and combined fractional diagonal chromatography (COFRADIC). The extended methodological repertoire promises fast and new insight into the intricate regulation network of the redox proteome of animals, bacteria, and plants.

Key words

2D gel electrophoresis - affinity chromatography - protein thiol - redox proteome - redox regulation - thioredoxin

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K.-J. Dietz

Faculty of Biology - W5-134
Bielefeld University

Universitätsstraße 25

33501 Bielefeld

Germany

eMail: karl-josef.dietz@uni-bielefeld.de

Editor: B. Schulz