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Plant Biol (Stuttg) 2004; 6(6): 654-663
DOI: 10.1055/s-2004-830383
Research Paper

Georg Thieme Verlag Stuttgart KG · New York

Gene Expression during Formation of Earlywood and Latewood in Loblolly Pine: Expression Profiles of 350 Genes

U. Egertsdotter1 , 5 , L. M. van Zyl2 , J. MacKay3 , G. Peter4 , M. Kirst2 , C. Clark2 , R. Whetten2 , R. Sederoff2
  • 1Georgia Institute of Technology, Institute of Paper Science and Technology, 500 10th Street, Atlanta, GA 30332, USA
  • 2Forest Biotechnology, North Carolina State University, 2500 Partners II, 840 Main Campus Drive, Centennial Campus, Raleigh, NC 27606, USA
  • 3Centre de Recherche en Biologie Forestière, Pavillon C. E. Marchand, Université Laval, Quebec G1K 7P4, Canada
  • 4School of Forest Resources and Conservation, University of Florida, Gainesville, FL 32611-0411, USA
  • 5Present address: Virginia Tech Department of Forestry, 230-A Cheatham Hall (0324), Blacksburg, VA 24061, USA
Further Information

Publication History

Received: May 6, 2004

Accepted: September 14, 2004

Publication Date:
29 November 2004 (online)

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Abstract

The natural variability of wood formation in trees affords opportunities to correlate transcript profiles with the resulting wood properties. We have used cDNA microarrays to study transcript abundance in developing secondary xylem of loblolly pine (Pinus taeda) over a growing season. The cDNAs were selected from a collection of 75 000 ESTs that have been sequenced and annotated (http://web.ahc.umn.edu/biodata/nsfpine/). Cell wall thickness and climatic data were related to earlywood and latewood formation at different time points during the growing season. Seventy-one ESTs showed preferential expression in earlywood or latewood, including 23 genes with no significant similarity to genes in GenBank. Seven genes involved in lignin synthesis were preferentially expressed in latewood. The studies have provided initial insights into the variation of expression patterns of some of the genes related to the wood formation process.

Key words

Microarray analysis - Pinus taeda - earlywood - latewood - transcript abundance.

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U. Egertsdotter

Virginia Tech Department of Forestry

230-A Cheatham Hall

Blacksburg, VA 24061

USA

Email: ulrika.egertsdotter@ipst.gatech.edu

Editor: M. Koornneef

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