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Plant Biol (Stuttg) 2002; 4(5): 567-575
DOI: 10.1055/s-2002-35435
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Speciation and Species Separation in Hordeum L. (Poaceae) Resolved by Discontinuous Molecular Markers[1]

H. A. El-Rabey 1 , A. Badr 2 , R. Schäfer-Pregl 3 , W. Martin 4 , F. Salamini 3
  • 1 Genetic Engineering and Biotechnology Research Institute, Minufiya University, PO Box 79, Sadat City, Egypt
  • 2 Department of Botany, Faculty of Science, Tanta University, Tanta, Egypt
  • 3 Max Planck Institut für Züchtungsforschung, Köln, Germany
  • 4 Heinrich Heine Universität, Institut für Botanik III, Düsseldorf, Germany
Further Information

Publication History

Received: February 8, 2002

Accepted: July 22, 2002

Publication Date:
15 November 2002 (online)

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Abstract

Amplified fragment length polymorphisms (AFLPs) were used to evaluate the capacity of discontinuous markers to reveal genetic structure within Hordeum, a challenging higher plant genus from the standpoint of natural systematics. Phylogenies of 63 accessions encompassing nine species from four Hordeum sections were inferred from polymorphisms scored at 600 loci. Phylogenies based on sequences from the nuclear internal transcribed spacer (ITS) region were constructed for comparison, but revealed severe sampling errors inherent to single genes. Although superior by virtue of providing genome-wide estimates of genetic similarity, the adoption of AFLPs in infrageneric studies requires caution. Comigrating AFLP bands studied here could be divided on the basis of band intensity variation into two types that are ∼ 100 % identical and < 40 % identical in DNA sequence, respectively, in infrageneric comparisons. Thus, the careful selection of AFLP bands to be analyzed bears heavily upon their phylogenetic utility. Within the H. murinum complex, which encompasses three morphologically distinct subspecies, AFLP data from 37 accessions reveal unexpected genetic differentiation between H. murinum, glaucum populations to the east and west of Alexandria (Egypt), suggesting the presence of allopatric speciation in the wake of human settlement.

Abbreviations

AFLPs: amplified fragment length polymorphisms

bp: base pair

Key words

AFLPs - phylogeny - speciation - hybridization

1 Sequences of all sequenced AFLP bands discussed in this paper are available from the web site http://www.mpiz-koeln.mpg.de/∼schaefer/hordeum/hord.html.

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1 Sequences of all sequenced AFLP bands discussed in this paper are available from the web site http://www.mpiz-koeln.mpg.de/∼schaefer/hordeum/hord.html.

W. Martin

Heinrich Heine Universität
Institut für Botanik III

Universitätsstraße 1
40225 Düsseldorf
Germany

Email: w.martin@uni-duesseldorf.de

Section Editor: U. Lüttge

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