High Frequency Plant Regeneration from Nodal Explants of Paulownia elongata

Abstract

High frequency of plant regeneration from Paulownia elongata was obtained on Murashige and Skoog (MS) medium and Woody Plant Medium (WPM), with appropriate supplements of growth regulators. Leaves, leaves with petioles, internodes and nodes excised from 3-month-old non-aseptically grown P. elongata were used as explants. The highest shoot regeneration efficiency (93.7 %) was obtained from the nodes of P. elongata on MS medium supplemented with 0.1 mg/ml naphthaleneacetic acid (NAA) and 1 mg/ml 6-benzylaminopurine (BAP). The highest root formation efficiency (100 %) from the regenerated shoots was obtained on WPM supplemented with 1 mg/ml indolebutyric acid (IBA). Rooted plantlets were transplanted to soil with a survival efficiency of almost 100 %. The regeneration system reported here could be useful for rapid multiplication of elite genotypes of P. elongata in a short period of time.

Abbreviations

BAP: 6-benzylaminopurine

IAA: indoleacetic acid

IBA: indolebutyric acid

MS: Murashige Skoog

NAA: naphthaleneacetic acid

WPM: Woody Plant Medium

Key words

Paulownia elongata - micropropagation - nodal explant - tissue culture

References

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Section Editor: A. M. C. Emons