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Plant Biol (Stuttg) 2000; 2(3): 306-315
DOI: 10.1055/s-2000-3700
Original Paper
Georg Thieme Verlag Stuttgart ·New York

Molecular Phylogenetics of Bromeliaceae: Evidence from trnL(UAA) Intron Sequences of the Chloroplast Genome

R. Horres 1 , G. Zizka 1 , G. Kahl 2 , K. Weising 3
  • 1 Botany/Paleobotany, Forschungsinstitut Senckenberg and Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt/M., Germany
  • 2 Plant Molecular Biology, Biozentrum, Johann Wolfgang Goethe-Universität Frankfurt, Frankfurt/M., Germany
  • 3 Plant Morphology and Systematics, Universität Kassel, Kassel, Germany
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Publication History

November 5, 1999

February 1, 2000

Publication Date:
31 December 2000 (online)

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Abstract

Phylogenetic relationships within Bromeliaceae were assessed by comparative sequencing of chloroplast trnL(UAA) intronic DNA. Sixty-seven taxa were analyzed, encompassing 62 species, two subspecies and two varieties from 32 bromeliad genera. One representative of Rapateaceae and two of Velloziaceae were used as outgroups. Introns were amplified by the polymerase chain reaction and conserved flanking primers. Amplification products were sequenced directly. Of 700 base pairs total trnL (UAA) intron size, 551 bp could be unambiguously aligned. Sequence divergence among ingroup taxa ranged from zero to 4.6 %. Phylogenetic reconstruction by parsimony analysis of 73 informative base substitutions yielded 1092 most parsimonious trees. The topology of the resulting strict consensus tree was only partially consistent with current classification schemes. Brocchinia and Ayensua grouped close together and formed a well-supported basal clade, suggesting a sister group relationship of both to the remainder of the family. The remaining ingroup species were divided into an unresolved polytomy of three lineages, comprising (1) all members of subfamily Tillandsioideae, (2) the three investigated Hechtia species, and (3) all Bromelioideae and the remaining Pitcairnioideae (except Brocchinia, Ayensua and Hechtia). The latter clade was split into another polytomy of five sublineages, one of which comprised all Bromelioideae. Whereas monophyly of Tillandsioideae and Bromelioideae was supported by trnL intron data, Pitcairnioideae as traditionally circumscribed are clearly polyphyletic. There is evidence that the genera Tillandsia, Vriesea, Guzmania, Abromeitiella, Deuterocohnia, Dyckia, Pitcairnia and Aechmea in the present circumscription are not monophyletic.

Key words

Bromeliaceae - phylogeny - subfamilial grouping - delimitation of genera - trnL intron - chloroplast genome

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G. Zizka

Botanisches Institut der Johann Wolfgang Goethe-Universität und Forschungsinstitut Senckenberg Senckenberganlage 25

60325 Frankfurt/M. Germany

Email: gzizka@sngkw.uni-frankfurt.de

Section Editor: M. Hasebe