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Thromb Haemost 1985; 54(04): 804-807
DOI: 10.1055/s-0038-1660137
Original Article
Schattauer GmbH Stuttgart

Soluble Crosslinked Fibrin(ogen) Polymers

Eberhard Selmayr
The Clinical Research Unit for Blood Coagulation and Thrombosis of the Max-Planck-Gesellschaft at the Justus-Liebig-Universitat, Gieβen, West Germany
,
Gert Müller-Berghaus
The Clinical Research Unit for Blood Coagulation and Thrombosis of the Max-Planck-Gesellschaft at the Justus-Liebig-Universitat, Gieβen, West Germany
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Further Information

Publication History

Received 29 March 1985

Accepted 16 September 1985

Publication Date:
19 July 2018 (online)

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Summary

The present study is concerned with the formation of fibrin-fibrinogen associations in the presence of FXIIIa while fibrinolysis was inhibited by aprotinin and EACA. SDS agarose-poly acryl-amide gel electrophoresis on 2.5% gels under non-reducing conditions and ultracentrifugation of the associations on urea-sucrose density gradients showed the formation of soluble crosslinked high molecular weight (HMW) fibrin-fibrinogen polymers with an estimated molecular size up to 10 times that of fibrinogen. After incubation of a mixture of 131I-fibrinogen (4 mg/ml) and 125I-desAA-fibrin (0.2 mg/ml) with FXIIIa (2 U/ml) for 1 h at 37ΰ C, about 5% of the fibrinogen and 80% of the fibrin was incorporated into the generated soluble HMW polymers. The detection of soluble crosslinked fibrin-fibrinogen polymers could be a useful diagnostic criterion for imminent DIC.

Key words

Soluble fibrin - Fibrinogen - FXIIIa - Prevention of clot formation
 

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