The Effect of Partial Splenectomy on Platelet Production in Mice

Summary

The effect of total and partial splenectomy on the number and production of circulating platelets was studied in mice. Five days after total and partial splenectomy the number of the peripheral blood platelets increased by 87% and 60%, respectively and the incorporation of ⁷⁵selenium methionine (⁷⁵Se-Met) into platelets was enhanced indicating that the thrombocytosis was due to increased platelet production. The results obtained by the two operative procedures were compared.

Since previous work from our laboratory has shown that a factor produced by splenic lymphocytes affects the platelet number in mice, it is suggested that the differences in the number of circulating platelets observed in animals after total and partial splenectomy may reflect a difference in the number of spleen lymphocytes removed.

Keywords

Partial splenectomy - Platelets - Thrombocytosis

• References

• 1 Kotilainen M. Platelet kinetics in normal subjects and haematological disorders with special reference to thrombocytopenia, and the role of the spleen. Scand J Haematol 1969; Suppl 5
• 2 Najean Y, Ardaillou N, Caen J, Larrieu M-J, Bernard J. Survival of radiochromium-labeled platelets in thrombocytopenias. Blood 1963; 22: 718-732
• 3 Baldini M. Idiopathic thrombocytopenic parpura. N Engl J Med 1966; 274: 1245-1251
• 4 Baldini M. Idiopathic thrombocytopenic purpura. N Engl J Med 1966; 274: 1360-1367
• 5 Harker LA. Thrombokinetics in idiopathic thrombocytopenic purpura. Br J Haematol 1970; 19: 95-99
• 6 Bessler H, Mandel EM, Djaldetti M. Role of the spleen and lymphocytes in regulation of the circulating platelet number in mice. J Lab Clin Med 1978; 91: 760-768
• 7 Penington DG. Assessment of platelet production with ⁷⁵Se selenomethionine. Br Med J 1969; 4: 782-784
• 8 Rak K. Effect of vincristine on platelet production in mice. Br J Haematol 1972; 22: 617-624
• 9 Karpatkin S, Strick N, Siskind GW. Detection of splenic anti-platelet antibody synthesis in idiopathic autoimmune thrombocytopenic purpura (ATP). Br J Haematol 1972; 23: 167-176