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Thromb Haemost 2000; 84(01): 32-38
DOI: 10.1055/s-0037-1613963
Commentary
Schattauer GmbH

Ontogeny of Beta 2 Glycoprotein I and Annexin V in Villous Placenta of Normal and Antiphospholipid Syndrome Pregnancies

Siobhán Donohoe
3   Dept. of Obstetrics, Liverpool Women’s Hospital, Liverpool, UK
,
John C. P. Kingdom
1   From the Departments of Haematology, Liverpool, UK
,
Ian J. Mackie
3   Dept. of Obstetrics, Liverpool Women’s Hospital, Liverpool, UK
,
Stephen Burrell
1   From the Departments of Haematology, Liverpool, UK
,
Siobhán Quenby
2   Obstetrics & Gynaecology, University College London, Liverpool, UK
,
Eric Jauniaux
1   From the Departments of Haematology, Liverpool, UK
,
Samuel J. Machin
3   Dept. of Obstetrics, Liverpool Women’s Hospital, Liverpool, UK
› Author AffiliationsWe wish to thank Tommyșs Campaign for funding this project, Dr J Amiral (Serbio, France) for supplying Annexin V reagents, Mr Keith Miller and Phillipa Munson, Immunohistochemistry, UCL for their valuable advice.
Further Information

Publication History

Received 25 March 1999

Accepted after resubmission 24 February 2000

Publication Date:
10 December 2017 (online)

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Summary

β2-glycoprotein I (β2GPI) and annexin V (AV) have been implicated in the pathophysiology of the antiphospholipid syndrome (APS). We investigated their placental expression in normal villous tissues throughout gestation; first trimester n = 10, early second trimester; n = 4, preterm; n = 5) and term; n = 7 and in APS (2 first trimester, 1 preterm and 8 term deliveries). β2GPI and AV were both expressed by the placenta from as early as seven weeks gestation and were colocalised to the syncytiotrophoblast. β2GPI staining was also observed in stromal cells, being present in phagocytic Hofbauer cells and surrounding newly formed fetal vessels in a perivascular pattern, from seven to seventeen weeks gestation. An abnormal morphological distribution of AV was noted in one first trimester APS placenta, and for β2GPI in a further first trimester placenta. When placental proteins were extracted from villous tissue, the concentration of AV/mg protein in term APS placentas (median, interquartile range) (aPS; 8.16, 7.87-9.72 µg/mg) was significantly higher (p <0.005) than normal term levels (normal; 2.47, 2.28-2.54 µg/mg). β2GPI increased with advancing gestation (first trimester; 0.93, 0.64-1.26 µg/mg, term; 3.67, 2.58-4.48 µg/mg) in normal pregnancy. Term APS placentas had a reduced β2GPI content (2.31, 1.87-2.49 µg/mg), p <0.05. The placental role of these proteins remains to be identified.

Keywords

β2-glycoprotein-I - annexin V - placenta - antiphospholipid syndrome (APS)
 

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