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DOI: 10.1055/s-0037-1613795
An Automated Heteroduplex Assay for the PlA Polymorphism of Glycoprotein IIb/IIIa, Multiplexed with Two Prothrombotic Genetic Markers[*]
Publikationsverlauf
Received
08. Juni 1999
Accepted after revision
15. Oktober 1999
Publikationsdatum:
27. Dezember 2017 (online)
![](https://www.thieme-connect.de/media/10.1055-s-00035024/200002/lookinside/thumbnails/10-1055-s-0037-1613795-1.jpg)
Summary
Screening for the PlA polymorphism has been made faster and simpler with the advent of heteroduplex technology. Simultaneous screening for three common prothrombotic polymorphisms PlA, factor V Leiden, and MTHFR(C677T) has been achieved with multiplex heteroduplex analysis. We describe a quick and simple method for PlA heteroduplex probe production. The probe was multiplexed with heteroduplex probes for MTHFR(C677T) and factor V Leiden polymorphisms in a one tube assay, allowing rapid automated genotyping of all three. This automated multiplex assay was applied to a cohort of 165 patients and showed excellent correlation with gel-based assays, both PAGE and RFLP. This approach will facilitate the analysis of multiple polymorphisms in complex disease in large populations.
* Supported by grants from the Wellcome Trust, the Higher Education Authority (HEA) and the Health Research Board (HRB) of Ireland. Dr. Murphy was a HRB Postdoctoral Fellow during the performance of this work.
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