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Planta Med 2012; 78(16): 1757-1760
DOI: 10.1055/s-0032-1315257
Letters
Georg Thieme Verlag KG Stuttgart · New York

The In Vitro Protective Effects of Curcumin and Demethoxycurcumin in Curcuma longa Extract on Advanced Glycation End Products-Induced Mesangial Cell Apoptosis and Oxidative Stress

Ji-ping Liu
1   Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang, P. R. China
,
Liang Feng
2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China
,
Mao-mao Zhu
2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China
,
Ru-Shang Wang
2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China
,
Ming-hua Zhang
2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China
,
Shao-ying Hu
2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China
,
Xiao-bin Jia
2   Key Laboratory of New Drug Delivery System of Chinese Materia Medica, Jiangsu Provincial Academy of Chinese Medicine, Nanjing, P. R. China
,
Jin-Jie Wu
3   Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Jiangsu Nanjing, P. R. China
› Author Affiliations
Further Information

Publication History

received 26 February 2012
revised 24 July 2012

accepted 26 July 2012

Publication Date:
24 August 2012 (online)

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Abstract

Curcuma longa L. (CLL), a traditional herbal medicine, has been widely used for the prevention of diabetic vascular complications in recent years. However, the protective effects of curcuminoids in CLL on the AGEs-induced damage to mesangial cell are not fully understood. In this present study, dihydroethidium, superoxide dismutase kit, malondialdehyde kit, and acridine orange/ethidium bromide staining methods were used to evaluate the activities of curcumin and demethoxycurcumin (10−11 − 10−9 M) on AGEs-induced oxidative stress and apoptosis, which were associated with the damage to mesangial cell. The results showed that these two compounds could significantly restore advanced glycation end products (AGEs)-induced apoptosis to normal levels (IC50 = 3.874 × 10−11 M for curcumin and IC50 = 6.085 × 10−11 M for demethoxycurcumin) and reduce remarkably reactive oxygen species generation in mesangial cell. Furthermore, curcumin and demethoxycurcumin dramatically elevated AGEs-decreased superoxide dismutase activity while significantly reducing AGEs-increased malondialdehyde content in cell culture supernatant. Our results suggest that both curcumin and demethoxycurcumin have a significant protective potential to the prevention of diabetic nephropathy.

Key words

Curcuma longa L. - Zingiberaceae - curcumin - demethoxycurcumin - diabetic nephropathy - anti-apoptosis - antioxidant

Supporting Information

 

  • References

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