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DOI: 10.1055/s-0031-1298373
© Georg Thieme Verlag KG Stuttgart · New York
Metabolic Profiling Assisted Quality Assessment of Rhodiola rosea Extracts by High-Performance Liquid Chromatography
Publikationsverlauf
received Dec. 5, 2011
revised February 16, 2012
accepted February 22, 2012
Publikationsdatum:
22. März 2012 (online)
![](https://www.thieme-connect.de/media/plantamedica/201207/lookinside/thumbnails/10.1055-s-0031-1298373-1.jpg)
Abstract
In this work, fast and sensitive high-performance liquid chromatography (HPLC) coupled with multivariate analysis was utilized to assist the quality assessment of Rhodiola rosea extracts (RREs). 131 peaks were separated and detected in RREs on a fused-core C18 column. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) of the chromatographic data demonstrated that 10 batches of RREs could be well-differentiated and categorized into three groups which were closely related to the origins of RREs. Partial least square-discriminant analysis (PLS-DA) showed that the quality differentiation might be explained by at least 6 components, in which rosavin was characterized by an external reference, rosiridine was identified by liquid chromatography–mass spectrometry (LC-MS), and the mass spectra of the others were provided. The observation that the level of rosavin was more relevant to the multivariate chromatographic data than the ones of salidroside and tyrosol, the other two components commonly used to standardize RREs, was confirmed by the PLS prediction models. Results of the present study not only indicated that rosavin was a rational marker to represent the quality of RREs, but also demonstrated the power of HPLC-based metabolic profiling in the quality assessment of herbal extracts.
Key words
metabolic profiling - Rhodiola rosea L. extracts - Crassulaceae family - quality assessment - high-performance liquid chromatography - fused-core column
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Prof. Fang Chen, Ph.D.
College of Life Sciences
Sichuan University No. 24 South Section 1
Yihuan Road, Chengdu
Sichuan 610065
China
eMail: chenfang@scu.edu.cn
Dr. Ke Lan, Ph.D.
Key Laboratory of Drug Targeting and Drug Delivery Systems
Ministry of Education
West China School of Pharmacy
Sichuan University No. 17
the 3rd section of Reminnan Road, Chengdu
Sichuan 610041
China
Telefon: +86 28 85 50 37 22
Fax: +86 28 85 50 37 22
eMail: lanwoco@scu.edu.cn