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Planta Med 1992; 58(1): 73-76
DOI: 10.1055/s-2006-961393
Paper

© Georg Thieme Verlag Stuttgart · New York

Shoot Tip Culture of Arnica montana for Micropropagation

Odile Conchou, Karin Nichterlein, Annelise Vömel
  • Institut für Pflanzenbau und Pflanzenzüchtung I, Justus-Liebig-Universität Giessen, Ludwigstr. 23, D(W)-6300 Giessen, Federal Republic of Germany
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Publikationsverlauf

1991

Publikationsdatum:
04. Januar 2007 (online)

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Abstract

Multiple shoots were regenerated from shoot tips of Arnica montana on MS and B5 media supplemented with BA (1 mg/l) and NAA (0.1 mg/l). Sections of 1-2 mm in length cultured from in vitro germinated seedlings regenerated 7.7 (mean) shoots on the MS medium, whereas sections cultured from greenhouse plants regenerated 9.0 (mean) shoots on the B5 medium within 6 weeks. Subsequent subcultures of shoots on the same media but without NAA resulted in similar or lower multiplication rates (1.6 to 3.1 in 3 weeks). Shoot development was promoted, whereas shoot initiation was simultaneously inhibited by the addition of activated charcoal to the media. Rooting was induced by culturing shoots from seedling as well as from greenhouse plant shoot tips on MS or B5 medium supplemented with NAA. The plantlets were transplanted into soil and grown successfully under greenhouse and field conditions.

Key words

Arnica montana - shoot tip culture - micro-propagation

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