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Planta Med 1999; 65(6): 549-552
DOI: 10.1055/s-1999-14087
Original Paper

Georg Thieme Verlag Stuttgart · New York

Isolation and Partial Characterization of Immunostimulating Polysaccharides from Imperata cylindrica

Véronique Pinilla, Bang Luu
  • Laboratoire de Chimie Organique des Substances Naturelles, UMR 7509-CNRS, Université Louis Pasteur, Strasbourg Cedex, France
Further Information

Publication History

October 30, 1998

March 14, 1999

Publication Date:
31 December 1999 (online)

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Abstract

The water-soluble crude extract prepared from Imperata cylindrica (Beauv.) was investigated for its immunomodulating activity. A set of polysaccharides with high molecular weights has been isolated by fractionation using gel filtration and anion-exchange chromatography. Each step of purification was monitored by bioassays. The presence of six monosaccharides has been established by chemical analysis. Quantitative analysis showed that the ratio of these monosaccharides differed from one polysaccharide to another. The crude extract as well as some of the purified polysaccharides enhance the proliferation of murine splenocytes.

Key words:

Imperata cylindrica - Poaceae - polysaccharide - extraction - immunomodulation

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