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Planta Med 2018; 84(11): 806-812
DOI: 10.1055/s-0043-124974
Natural Product Chemistry and Analytical Studies
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Effective Antimalarial Activities of α-Hydroxy Diynes Isolated from Ongokea gore

Joséphine Kankolongo Ntumba
1   Department of Chemistry, Faculty of Sciences, University of Kinshasa, Democratic Republic of Congo
,
Christian Muamba Tshiongo
1   Department of Chemistry, Faculty of Sciences, University of Kinshasa, Democratic Republic of Congo
,
Michel Ngoma Mifundu
1   Department of Chemistry, Faculty of Sciences, University of Kinshasa, Democratic Republic of Congo
,
Raphäel Robiette
2   Institute of Condensed Matter and Nanosciences, Université Catholique de Louvain, Belgium
,
Kalulu Muzele Taba
1   Department of Chemistry, Faculty of Sciences, University of Kinshasa, Democratic Republic of Congo
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 03. Mai 2017
revised 28. November 2017

accepted 12. Dezember 2017

Publikationsdatum:
04. Januar 2018 (online)

    Lizenzen und Reprints

Abstract

Three diynes, octadec-17-ene-9,11-diynoate ethyl (1), 8-hydroxy-octadeca-13,17-diene-9,11-diynoate ethyl (2), and 8-hydroxy-octadec-13-ene-9,11-diynoate ethyl (3), were isolated from Ongokea gore seed oil. The structure assignment of these three compounds was based according to chemical and spectroscopic data. They were screened against Plasmodium falciparum, the parasite that causes malaria. In vitro micro-test (Mark III, supported by the World Health Organization) was developed to assess the response of P. falciparum to the isolated three compounds, and statistical analysis were performed for determination of the concentration that inhibits 50% of the parasite maturation (IC50). Two of the three diynes (2 and 3) showed a very effective in vitro antimalarial activity with an IC50 of 4.5 and 1.7 µM, respectively. Compound 3 exhibited better activity than quinine (IC50 1.9 µM), the drug reference, while compound 1 had no antimalarial activity (IC50 > 125 µM). In the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) cytotoxicity screening, all compounds showed no toxicity (mean IC50 of 90 µM for each compound).

Key words

diynes - Ongokea gore - Olacaceae - in vitro - cytotoxicity

Supporting Information

    IC50 determinations as dose-response curves as well as the mathematical method of determination of the values are available as Supporting Information.

  • Supporting Information
 

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