Download PDF
Thromb Haemost 1961; 6(02): 248-253
DOI: 10.1055/s-0038-1654557
Originalarbeiten — Original Articles — Travaux Originaux
Schattauer GmbH

The Relation between a Naturally Occuring Inhibitor and Prothrombin in Intermediate Product I Formation

E. T Yin
1   Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) Kantonsspital, University of Zürich, Switzerland
,
J. N Shanberge   *
1   Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) Kantonsspital, University of Zürich, Switzerland
,
F Duckert
1   Coagulation Laboratory (Prof. F. Koller) of the Department of Medicine (Prof. P. H. Rossier) Kantonsspital, University of Zürich, Switzerland
› Author Affiliations
Further Information

Publication History

Publication Date:
12 July 2018 (online)

    Permissions and Reprints

Summary

1. A method for the separation from clotting factors and the concentration of an inhibitor of intermediate product I present in adsorbed plasma and adsorbed serum is described.

2. This inhibitor is not only stable but also activated at 37° C. At 4° C it loses activity.

3. The inhibitory effect on the formation of intermediate product I is counteracted by small amounts of prothrombin or thrombin.

4. After addition of inhibitor prothrombin is necessary for the formation of intermediate product I in the purified system.

5. The discrepancy, in regard to the need for prothrombin (or thrombin) between the purified and the non-purified system can be explained by the relative amounts of inhibitor in the two systems.

* Investigation carried out during the tenure of a Special Fellowship from the American Heart Institute, United States Public Health Service.


 

  • References

  • (1) Duckert F, Koller F, Matter M. Purification and Physiological Properties of Factor VII from Plasma and Serum. Separation from Prothrombin. Proc. Soc. exp. Biol. (N. Y.) 82: 259 1953;
    CrossrefPubMedGoogle Scholar
  • (2) Duckert F, Yin E. T, Straub W. Separation and Purification of the Blood Clotting Factors by means of Chromatography and Electrophoresis. Preparative Methods. Protides of the Biological Fluids. Proc. 8th Colloquium, Bruges 1960. 41 Elsevier; Amsterdam: 1961
    Google Scholar
  • (3) Mayer G, Mayer S, Waitz R. The Inhibitory Action of Adsorbed Undiluted (Normal or Pathological) Plasma on the Thromboplastin Generation Test. Thromb. Diath. haem. 4: 349 1960;
    PubMedGoogle Scholar
  • (4) Samama M, Terrier E, Leroux M. E, Bilski-Pasquier G, Duhamel G, Marchai G. La Thrombo-dynamographie dans l’Etude des Inhibiteurs de la. Coagulation. Sang 30: 329 1959;
    PubMedGoogle Scholar
  • (5) Shanberge J. N, Duckert F. The Influence of Contact and Prothrombin on Intermediate Product I Formation. Thromb. Diath. haem. 6: 235 1961;
    PubMedGoogle Scholar
  • (6) Yin E. T, Duckert F. Purification of Factor IX or of Its Precursor. Isolation of a Hageman-PTA Fraction. Thromb. Diath. haem. 6: 215 1961;
    PubMedGoogle Scholar
  • (7) Yin E. T, Duckert F. The Formation of Intermediate Product I in a Purified System. The Rôle of Factor IX or of Its Precursor and of a Hageman Factor — PTA Fraction. Thromb. Diath. haem. 6: 224 1961;
    PubMedGoogle Scholar