CHEMICAL STUDIES OF A FIXED OIL, TERPENOIDS AND OTHER COMPOUNDS FROM THE PODS OF HETEROPHRAGMA ADENOPHYLLUM

Abstract

The fixed oil from the pods of Heterophragma adenophyllum Seem, has been studied for its fattty acid composition by the combined application of solid–liquid countercurrent distribution of urea inclusion complexes of fatty acids after being segregated into solid and liquid fractions by lead–salt method as well as of the mixed acids and mixed methyl esters, quantitative column chromatography and , spectrophotometry. The acids were identified by uni– and two dimensional reversed phase paper partition and thin–layer Chromatographie techniques. The fatty acids as found by different techniques were: lauric acid, 1.23–1.79; myristic acid, 1.21–1.60; palmitic acid, 2.91–3.24; stearic acid, 7.66–8.50; arachidic acid, 0.94–1.39; oleic acid, 16.88–19.55; linoleic acid, 30.02–31.22 and linolenic acid 35.29–38.79 %. The chief ingredients of unsaponofiable matter were found to be: (I) octacosane or an equimolecular mixture of C₂₇ and C₂g paraffins, (II) unidentified C₆H₅–C₁₈H₃₇ hydrocarbon, (III) myricyl alcohol, (IV) friedelin, (v) β–amyrin, (VI) unidentified triterpenoid, C₃₀H₅₀O₂ (m. p. 230–31°) and (VII) β–sitosterol.