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DOI: 10.1055/a-1953-0369
The Anti-inflammatory Effects of Isoflavonoids from Radix Astragali in Hepatoprotective Potential against LPS/D-gal-induced Acute Liver Injury
Supported by: National Natural Science Foundation of China 81771167Supported by: National Natural Science Foundation of China U1608282
Abstract
Radix Astragali (RA) is an important Traditional Chinese Medicine widely used in the treatment of various diseases, such as pneumonia, atherosclerosis, diabetes, kidney and liver fibrosis. The role of isoflavonoids from RA in the treatment of liver injury remains unclear. The study aimed to explore hepatoprotective and anti-inflammatory effects of isoflavonoids from Astragalus mongholicus. Network pharmacological analysis showed that RA had a multi-target regulating effect on alleviating liver injury and inhibiting inflammation through its active ingredients, among which isoflavones were closely related to its key molecular targets. The anti-inflammatory and liver protection effects of isoflavonoids of RA were investigated using lipopolysaccharide (LPS)-induced RAW 264.7 cells in vitro and LPS/D-galactosamine (D-gal)-induced acute liver injury mice in vivo. The experimental results showed that methylnissolin (ML) and methylnissolin-3-O-β-D-glucoside (MLG) presented more notable anti-inflammatory effects. Both of them suppressed the release of pro-inflammatory cytokines, such as iNOS, COX-2, IL-1β, IL-6, and TNF-α in LPS-stimulated RAW 264.7 cells. In vivo investigation demonstrated that ML markedly meliorated liver injury in LPS/D-gal-induced mice. Western blot results revealed that ML and MLG down-regulated the expression of proinflammatory cytokines via NF-κB signaling pathway. The isoflavonoids, methylnissolin (ML), and methylnissolin-3-O-β-D-glucoside (MLG), play a vital role in the hepatoprotective and anti-inflammatory effects of RA.
Key words
Astragalus mongholicus - Fabaceae - isoflavonoids - anti-inflammation - LPS/D-gal-induced acute liver injury - NF-κB signaling pathway - network pharmacologySupporting Information
- Supporting Information
List of Abbreviations, extraction and isolation of A. mongholicus, NMR spectra of compounds 1–8, as well as the primers for qRT-PCR can be found in Supplementary Information.
Publication History
Received: 10 April 2022
Accepted after revision: 12 September 2022
Article published online:
12 December 2022
© 2022. Thieme. All rights reserved.
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