Planta Med 2022; 88(03/04): 300-312
DOI: 10.1055/a-1646-2959
Biological and Pharmacological Activity
Original Papers

Efficacy of Norway Spruce Ointments and Bacterial and Fungal Alterations in the Treatment of Castration Wounds in Piglets

Désirée Prokop
1   Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
,
Joachim Spergser
2   Institute of Microbiology, Department of Pathobiology, University of Veterinary Medicine, Vienna, Austria
,
Werner Hagmüller
3   Institute of Organic Farming and Farm Animal Biodiversity, HBLFA Raumberg-Gumpenstein, Thalheim/Wels, Austria
,
Alexander Tichy
4   Bioinformatics and Biostatistics Platform, Department of Biomedical Sciences, University of Veterinary Medicine, Vienna, Austria
,
1   Institute of Animal Nutrition and Functional Plant Compounds, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine, Vienna, Austria
› Author Affiliations

Abstract

This study aimed to evaluate the efficacy of Norway spruce ointments on wound healing of castration wounds in piglets. This study included 95 male pigs randomly divided into five treatment groups: Norway spruce balm (Vulpuran), Norway spruce resin (Abilar), pork lard (ointment base of Vulpuran), no treatment (negative control) and antibiotic blue spray (Cyclo spray, positive control). Wound healing parameters (such as healing time, wound size, reddening of wound edges and surrounding, swelling, secretion and wound contamination), microbiological status and the haptoglobin level as an inflammation parameter were investigated. In the Norway spruce groups, some positive effects on wound healing parameters were found. In the first 6 days of treatment, Abilar or Vulpuran showed the smallest means of wound areas, and at the end of the study (day 15 + 17), the highest rates of completely closed wounds compared to the other groups. Vulpuran treatment led to significantly lower wound secretion (p = 0.003) and wound contamination (p = 0.015) than the untreated control did. Furthermore, the microbiological status was determined using MALDI-TOF-MS and partial 16S rRNA gene sequencing at different days of treatment. A comparison of the five treatment groups on day 3 revealed that Norway spruce led to the lowest rate of wounds colonised with fungi, mainly classified into genus Candida, (Abilar 77%, Vulpuran 70%) in comparison with blue spray (89%), lard (100%) and untreated control (100%). Fungi could only be detected in one of the 13 samples treated with Vulpuran on day 8, which nearly reached significance (p = 0.055).

Supporting Information



Publication History

Received: 27 February 2021

Accepted after revision: 15 September 2021

Article published online:
08 October 2021

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  • References

  • 1 Enoch S, Leaper DJ. Basic science of wound healing. Surgery 2008; 26: 31-37
  • 2 Velnar T, Bailey T, Smrkolj V. The wound healing process: an overview of the cellular and molecular mechanisms. J Int Med Res 2009; 37: 1528-1542
  • 3 Cavanagh D, Beazley J, Ostapowicz F. Radical operation for carcinoma of the vulva. A new approach to wound healing. J Obstet Gynaecol Br Commonw 1970; 77: 1037-1040
  • 4 Mandal MD, Mandal S. Honey: Its medicinal property and antibacterial activity. Asian Pac J Trop Biomed 2011; 1: 154-160
  • 5 Irish J, Carter DA, Shokohi T, Blair SE. Honey has an antifungal effect against Candida species. Med Mycol 2006; 44: 289-291
  • 6 Moore OA, Smith LA, Campbell F, Seers K, McQuay HJ, Moore RA. Systematic review of the use of honey as a wound dressing. BMC Complement Altern Med 2001; 1: 2
  • 7 Dunford C, Cooper R, Molan P, White R. The use of honey in wound management. Nurs Stand 2000; 15: 63-68
  • 8 Oryan A, Zaker SR. Effects of topical application of honey on cutaneous wound healing in rabbits. Zentralbl Veterinaermed A 1998; 45: 181-188
  • 9 Ghelardini C, Galeotti N, Salvatore G, Mazzanti G. Local anaesthetic activity of the essential oil of Lavandula angustifolia. Planta Med 1999; 65: 700-703
  • 10 Hajhashemi V, Ghannadi A, Sharif B. Anti-inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia Mill. J Ethnopharmacol 2003; 89: 67-71
  • 11 Cavanagh HMA, Wilkinson JM. Biological activities of lavender essential oil. Phytother Res 2002; 16: 301-308
  • 12 Lusby PE, Coombes AL, Wilkinson JM. A comparison of wound healing following treatment with Lavandula x allardii honey or essential oil. Phytother Res 2006; 20: 755-757
  • 13 Nada A, Abu-Ahmed H, Khafaga A, El-Kammar M. Clinical and histopathological evaluation of the effectiveness of lavender oil compared with black seed oil, ostrich oil and cod liver oil on the second intention wound healing in dogs. AJVS 2015; 46: 57
  • 14 Sipponen A, Jokinen JJ, Sipponen P, Papp A, Sarna S, Lohi J. Beneficial effect of resin salve in treatment of severe pressure ulcers: a prospective, randomized and controlled multicentre trial. Br J Dermatol 2008; 158: 1055-1062
  • 15 Sipponen A, Kuokkanen O, Tiihonen R, Kauppinen H, Jokinen JJ. Natural coniferous resin salve used to treat complicated surgical wounds: pilot clinical trial on healing and costs. Int J Dermatol 2012; 51: 726-732
  • 16 Jokinen JJ, Sipponen A. Refined spruce resin to treat chronic wounds: Rebirth of an old folkloristic therapy. Adv Wound Care 2016; 5: 198-207
  • 17 Sipponen A, Rautio M, Jokinen JJ, Laakso T, Saranpaa P, Lohi J. Resin-salve from Norway spruce – A potential method to treat infected chronic skin ulcers?. DML 2007; 1: 143-145
  • 18 Rautio M, Sipponen A, Peltola R, Lohi J, Jokinen JJ, Papp A. et al. Antibacterial effects of home-made resin salve from Norway spruce (Picea abies). APMIS 2007; 115: 335-340
  • 19 Sipponen A, Peltola R, Jokinen JJ, Laitinen K, Lohi J, Rautio M. et al. Effects of Norway spruce (Picea abies) resin on cell wall and cell membrane of Staphylococcus aureus. Ultrastruct Pathol 2009; 33: 128-135
  • 20 Rautio M, Sipponen A, Lohi J, Lounatmaa K, Koukila-Kähkölä P, Laitinen K. In vitro fungistatic effects of natural coniferous resin from Norway spruce (Picea abies). Eur J Clin Microbiol Infect Dis 2012; 31: 1783-1789
  • 21 Sipponen P, Sipponen A, Lohi J, Soini M, Tapanainen R, Jokinen JJ. Natural coniferous resin lacquer in treatment of toenail onychomycosis: An observational study. Mycoses 2013; 56: 289-296
  • 22 Auvinen T, Tiihonen R, Soini M, Wangel M, Sipponen A, Jokinen JJ. Efficacy of topical resin lacquer, amorolfine and oral terbinafine for treating toenail onychomycosis. A prospective, randomized, controlled, investigator-blinded, parallel-group clinical trial. Br J Dermatol 2015; 173: 940-948
  • 23 Holmbom T, Reunanen M, Fardim P. Composition of callus resin of Norway spruce, Scots pine, European larch and douglas fir. Holzforschung 2008; 62: 444
  • 24 Goels T, Eichenauer E, Langeder J, Hoeller F, Sykora C, Tahir A. et al. Norway spruce balm. Phytochemical composition and ability to enhance re-epithelialization in vitro . Planta Med 2020; 86: 1080-1088
  • 25 Heegaard PM, Stockmarr A, Piñeiro M, Carpintero R, Lampreave F, Campbell FM, Eckersall PD, Toussaint MJ, Gruys E, Sorensen NS. Optimal combinations of acute phase proteins for detecting infectious disease in pigs. Vet Res 2011; 42: 50
  • 26 Hennig-Pauka I, Menzel A, Boehme TR, Schierbaum H, Ganter M, Schulz J. Haptoglobin and C-reactive protein-non-specific markers for nursery conditions in swine. Front Vet Sci 2019; 6: 92
  • 27 Sipponen A, Laitinen K. Antimicrobial properties of natural coniferous rosin in the European pharmacopoeia challenge test. APMIS 2011; 119: 720-724
  • 28 Sipponen A, Jokinen JJ, Lohi J. Resin salve from the Norwegian spruce tree: A ‘novel’ method for the treatment of chronic wounds. J Wound Care 2007; 16: 72-74
  • 29 Spergser J, Loncaric I, Tichy A, Fritz J, Scope A. The cultivable autochthonous microbiota of the critically endangered northern bald ibis (Geronticus eremita). PLoS One 2018; 13: e0195255
  • 30 Lane DJ. 16S/23S rRNA Sequencing. In: Stackebrandt E, Goodfellow M. eds. Nucleic Acid Techniques in bacterial Systematics. Chichester: Wiley; 1991: 115-175
  • 31 Yoon SH, Ha SM, Kwon S, Lim J, Kim Y, Seo H, Chun J. Introducing EzBioCloud. A taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67: 1613-1617
  • 32 Stackebrandt E, Ebers J. Taxonomic parameters revisited. Tarnished gold standards. Microbiol Today 2006; 8: 6-9
  • 33 Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44: 846-849