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Thromb Haemost 1966; 15(01/02): 052-068
DOI: 10.1055/s-0038-1649411
Originalarbeiten — Original Articles — Travaux Originaux
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Incorporation of Labelled Nucleotides and Aggregation of Human Blood Platelets[*]

E. W Salzman   **)
1   Department of Surgery, Harvard Medical School, and the Surgical Services, Massachusetts General Hospital, Boston
,
D. A Chambers
1   Department of Surgery, Harvard Medical School, and the Surgical Services, Massachusetts General Hospital, Boston
,
Lena L. Neri
1   Department of Surgery, Harvard Medical School, and the Surgical Services, Massachusetts General Hospital, Boston
› Author Affiliations
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Publication History

Publication Date:
27 June 2018 (online)

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Summary

Addition of ADP-8-C14 to platelet-rich plasma results in platelet radioactivity through a series of temperature-dependent reactions involving stepwise dephosphorylation of the nucleotide in plasma and then incorporation of the adenosine residue by the platelet, where it participates as a precursor in synthesis of platelet adenine nucleotides. This sequence of events, which requires a heat-labile nondialysable plasma factor, is blocked by 0.01 M KCN, by EDTA, by arginine methyl ester, by adenosine, and by AMP.

ADP-induced platelet aggregation does not require the reactions outlined above and proceeds without ADP - breakdown and without binding of the ADP- molecule to the platelet. A possible mechanism of this phenomenon is suggested.

*) Presented in part before the American Physiological Society, April, 1965, and published in abstract form [Fed. Proc. 24: 260 (1965)]. Supported by grants from the National Heart Institute of the U.S. Public Health Service and the American Heart Association.


**) Fellow of the Medical Foundation, Inc.


 

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