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DOI: 10.1055/a-1828-2671
Euryachincoside, a Novel Phenolic Glycoside with Anti-Hepatic Fibrosis Activity from Eurya chinensis
Supported by: National Natural Science Foundation of China 81903509
Abstract
Eurya chinensis has been recorded as a folk medicine traditionally used for treatment of a variety of symptoms. However, the phytochemical and pharmacological investigations of this plant are still scarce. A novel phenolic glycoside named Euryachincoside (ECS) was isolated by chromatographic separation from E. chinensis, and its chemical structure was identified by analysis of HRMS and NMR data. Its anti-hepatic fibrosis effects were evaluated in both HSC-T6 (rat hepatic stellate cells) and carbon tetrachloride (CCl4)-induced mice with Silybin (SLB) as the positive control. In an in vitro study, ECS showed little cytotoxicity and inhibited transforming growth factor-beta (TGF-β)-induced Collagen I (Col1) along with alpha-smooth muscle actin (α-SMA) expressions in HSC-T6. An in vivo study suggested ECS significantly ameliorated hepatic injury, secretions of inflammatory cytokines, and collagen depositions. Moreover, ECS markedly mediated Smad2/3, nuclear factor kappa B (NF-κB) and nuclear factor erythroid 2-related factor 2 (Nrf2) signaling pathways both in vitro and vivo. These present findings confirmed that ECS is a novel phenolic glycoside from E. chinensis with promising curative effects on hepatic fibrosis, and its mechanisms may include decreasing extracellular matrix accumulation, reducing inflammation and attenuating free radicals via Smad2/3, NF-κB and Nrf2 signaling pathways, which may shed light on the exploration of more effective phenolic glycoside-based anti-fibrotic agents.
Supporting Information
- Supporting Information
HR-ESI-MS (Fig. 1S), 1H and 13C NMR (Fig. 2S and 3S), 1H-1H COSY (Fig. 4S), HSQC (Fig. 5S), HMBC (Fig. 6S), IR (Fig. 7S), UV (Fig. 8S) and Chromatogram of L-glucose, D-glucose and hydrolyzed ECS derivatized by L-cysteine methyl ester hydrochloride and aryl isothiocyanate (Fig. 9S) spectra of ECS and PCR primers sequences of Col1, α-SMA and GAPDH (Table 1S) are available in the Supporting Information. Also the instruments and materials for the purification of the compound from the title plant and for the spectroscopic measurements of the purified compound are detailed in the Supporting Information.
Publication History
Received: 04 January 2022
Accepted after revision: 14 April 2022
Accepted Manuscript online:
19 April 2022
Article published online:
23 January 2023
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